Counter-current chromatography based analysis of synergy in an anti-tuberculosis ethnobotanical
Counter-current chromatography based analysis of synergy in an anti-tuberculosis ethnobotanical
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DOI：
10.1016/j.chroma.2007.01.127
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发表时间：
2007-06-01
影响因子：
4.1
通讯作者：
Pauli, Guido F.
中科院分区：
文献类型：
--
作者：
Inui, Taichi;Wang, Yuehong;Pauli, Guido F.
对阿拉斯加民族植物学植物刺人参的粗提物进行逆流色谱（CCC），并利用体外抗结核功效模型评价所选活性区域的协同效应。CCC作为一种无载体的高分辨率分离方法，用于防止潜在的不可逆吸附到固体固定相。分别用微孔板阿利兰法和等效线法测定生物活性和消除剂量效应依赖性误差。将CCC、生物测定和等效线法相结合，观察到显著的增效作用。在整个极性范围内，具有0.44和0.81之间的分布常数的级分显示出最大的协同增强，重组级分的效力增加了108%。(C)2007 Elsevier B.V.保留所有权利。
The crude extract of an Alaskan ethnobotanical plant, Oplopanax horridus, was subjected to counter-current chromatography (CCC), and the selected active regions were evaluated for their synergistic effects with an in vitro model of anti-tubercular efficacy. CCC as a support-free high-resolution separation method was employed to preclude potential irreversible absorption to a solid stationary phase. The microplate Alamar blue assay and the isobole method were used to measure the biological activity and eliminate dose-response dependent errors, respectively. Using the combination of CCC, bioassay and isobole method, significant synergistic effects were observed. Among the entire polarity range, fractions with distribution constant between 0.44 and 0.81 showed the most synergistic enhancement with an increase in potency by 108% for the recombined fractions. (C) 2007 Elsevier B.V. All rights reserved.