Expression pattern of zinc-finger transcription factor Odd-skipped related 2 in murine development and neonatal stage
Expression pattern of zinc-finger transcription factor Odd-skipped related 2 in murine development and neonatal stage
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DOI：
10.1016/j.gep.2013.06.007
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发表时间：
2013-12-01
影响因子：
1.2
通讯作者：
Amano, Atsuo
中科院分区：
文献类型：
--
作者：
Kawai, Shinji;Michikami, Ikumi;Amano, Atsuo
Odd-skipped 基因首先被鉴定为果蝇配对规则锌指转录因子，在果蝇发育中发挥着重要作用。哺乳动物同源物奇跳相关 2 (Osr2) 调节小鼠胚胎的四肢、牙齿和肾脏发育。然而，Osr2在新生儿发育过程中的详细表达模式仍不清楚。在本研究中，我们使用 qPCR 和原位杂交方法研究了小鼠新生儿和胚胎组织中的 Osr2 表达模式。首先，我们通过qPCR检查了Osr2的组织分布，发现它在子宫中高表达，在睾丸、小肠和前列腺中中等表达。在眼睛、肾脏、胎盘、肺、胸腺、淋巴结、胃和骨骼肌组织以及所有胚胎阶段也发现了这种表达。另一方面，Osr2 在大脑、心脏、肝脏或脾脏样本中不表达。接下来，我们使用原位杂交检查了 Osr2 的组织定位。 Osr2 在 E13.5 的颅面部区域被发现，在牙胚间充质以及 E17.5 的肾小体中显着表达。在新生儿组织中，Osr2在牙乳头、牙囊、哈德氏腺、鼻骨、眼睑真皮、滑膜关节和胫骨皮下组织中表达。我们的研究结果表明，Osr2 在生殖系统器官中发挥作用，例如子宫、睾丸、前列腺、胎盘和卵巢。此外，根据其在肾脏、哈德氏腺、眼睑真皮和胫骨皮下组织中的表达，该转录因子可能参与激素合成和功能。总之，我们的结果证明了 Osr2 在出生后发育和胚胎发生中的作用。 (C) 2013 Elsevier B.V. 保留所有权利。
The Odd-skipped gene, first identified as a Drosophila pair-rule zinc-finger transcription factor, plays an important role in Drosophila development. The mammalian homolog, Odd-skipped related 2 (Osr2), regulates limb, tooth, and kidney development in mouse embryos. However, the detailed expression pattern of Osr2 during neonatal development remains unclear. In this study, we investigated Osr2 expression patterns in mouse neonatal and embryo tissues using qPCR and in situ hybridization methods. First, we examined the tissue distribution of Osr2 by qPCR, and found it to be highly expressed in the uterus and moderately in the testes, small intestine, and prostate. That expression was also found in eye, kidney, placenta, lung, thymus, lymph node, stomach, and skeletal muscle tissues, and in all embryonic stages. On the other hand, Osr2 was not expressed in brain, heart, liver, or spleen samples. Next, we examined the tissue localization of Osr2 using in situ hybridization. Osr2 was found in the craniofacial region on E13.5, with notable expression in dental germ mesenchyme as well as the renal corpuscle on E17.5. As for neonatal tissues, Osr2 was expressed in the dental papilla, dental follicle, Harderian gland, nasal bone, eyelid dermis, synovial joint, and tibial subcutis. Our findings suggest that Osr2 functions in reproductive system organs, such as the uterus, testes, prostate, placenta, and ovaries. Furthermore, based on its expression in kidney, Harderian gland, eyelid dermis, and tibial subcutis tissues, this transcription factor may be involved in hormone synthesis and function. Together, our results demonstrate the role of Osr2 in postnatal development and embryogenesis. (C) 2013 Elsevier B.V. All rights reserved.