Mutation and selection explain why many eukaryotic centromeric DNA sequences are often A + T rich.
Mutation and selection explain why many eukaryotic centromeric DNA sequences are often A + T rich.
复制标题
DOI：
10.1093/nar/gkab1219
复制
发表时间：
2022-01-11
影响因子：
14.9
通讯作者：
Brown WRA
中科院分区：
文献类型：
--
作者：
Barbosa AC;Xu Z;Karari K;Williams W;Hauf S;Brown WRA
我们利用染色体工程技术，在分裂酵母裂糖酵母（Schizosaccharomyces pombe）的两种不同菌株中，用不同的原着丝粒测试序列替换原着丝粒DNA，发现无论是人工合成的还是细菌来源的富含A + T的DNA，都将在该物种中发挥着着丝粒的作用。利用基因组大小作为有效种群大小（Ne）的逆函数，我们还发现43种动物、真菌和酵母（Opisthokonta）物种的着丝粒DNA的相对a + T含量与Ne呈比例关系。这表明，在大多数这些物种中，着丝粒DNA的A + T含量是由选择和突变之间的平衡决定的。结合实验结果和进化分析，我们可以得出结论，在大多数Opisthokonta物种中，几乎任何序列的富含A + T的DNA都可以作为着丝粒。许多G/C到A/T的替换不太可能被选择，这可能有助于着丝粒DNA的快速进化。我们还表明，新着丝粒序列不仅仅是天然着丝粒DNA的弱版本，并且表明除了天然着丝粒所需的因子外，新着丝粒还需要用于其繁殖或建立的因子。
We have used chromosome engineering to replace native centromeric DNA with different test sequences at native centromeres in two different strains of the fission yeast Schizosaccharomyces pombe and have discovered that A + T rich DNA, whether synthetic or of bacterial origin, will function as a centromere in this species. Using genome size as a surrogate for the inverse of effective population size (Ne) we also show that the relative A + T content of centromeric DNA scales with Ne across 43 animal, fungal and yeast (Opisthokonta) species. This suggests that in most of these species the A + T content of the centromeric DNA is determined by a balance between selection and mutation. Combining the experimental results and the evolutionary analyses allows us to conclude that A + T rich DNA of almost any sequence will function as a centromere in most Opisthokonta species. The fact that many G/C to A/T substitutions are unlikely to be selected against may contribute to the rapid evolution of centromeric DNA. We also show that a neo-centromere sequence is not simply a weak version of native centromeric DNA and suggest that neo-centromeres require factors either for their propagation or establishment in addition to those required by native centromeres.
登录
查看更多内容
影响因子：
16.6
作者：
Guo LY;Allu PK;Zandarashvili L;McKinley KL;Sekulic N;Dawicki-McKenna JM;Fachinetti D;Logsdon GA;Jamiolkowski RM;Cleveland DW;Cheeseman IM;Black BE
通讯作者：
Black BE
DOI：
10.1073/pnas.1700764114
发表时间：
2017-07-18
影响因子：
11.1
作者：
Diner, Rachel E.;Nodding, Chari M.;Weyman, Philip D.
通讯作者：
Weyman, Philip D.
影响因子：
4.5
作者：
Catania S;Pidoux AL;Allshire RC
通讯作者：
Allshire RC
DOI：
10.1073/pnas.1216934111
发表时间：
2014-02-04
影响因子：
11.1
作者：
Brown, William R. A.;Thomas, Geraint;Loose, Matthew W.
通讯作者：
Loose, Matthew W.
DOI：
10.1016/j.cub.2014.08.017
发表时间：
2014-10-06
期刊：
Current biology : CB
影响因子：
--
作者：
Chmátal L;Gabriel SI;Mitsainas GP;Martínez-Vargas J;Ventura J;Searle JB;Schultz RM;Lampson MA
通讯作者：
Lampson MA