Effect of exhaustive exercise stress on the cytokine response.
Effect of exhaustive exercise stress on the cytokine response.
复制标题
力竭运动应激对细胞因子反应的影响。
DOI：
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发表时间：
1997
影响因子：
4.1
通讯作者：
H. Northoff
中科院分区：
文献类型：
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作者：
C. Weinstock;D. König;Regine Harnischmacher;J. Keul;A. Berg;H. Northoff
15名运动员在运动负荷测试前24小时、后1小时和后20小时接受调查（平均持续时间68分钟）。用脂多糖（LPS）、魔豆蛋白A （Con A）或植瘤凝集素（PHA）刺激全血细胞培养，在血清、尿液和上清液中检测细胞因子。血清和尿液1小时后，白细胞介素6 （IL-6）和可溶性IL-2受体（sIL-2R）水平升高。运动后血清中tnf - α升高，尿液中IL-2降低。所有其他血清和尿液测试（包括ifn - γ）均为阴性或阴性结果。在细胞培养中，lps诱导的炎症细胞因子tnf - α、IL-1和IL-6的释放在运动后1小时被抑制。运动后1 h， con - a诱导和lps诱导的ifn - γ释放和pha诱导的IL-2释放均被抑制。相比之下，con - a诱导的IL-2释放在跑步后轻度增加。我们的结论是，这里描述的强度和持续时间的运动导致免疫系统的激活，这是立即反调节。运动20小时后，大多数观察到的变化都回到了运动前的水平，这表明这种抑制性反调节的持续时间很短。
Fifteen athletes were investigated 24 h before, 1 h after, and 20 h after an exhaustive exercise stress test (mean duration 68 min). Testing for cytokines was done in serum, urine, and the supernatants of whole blood cell cultures, which were stimulated with lipopolysaccharide (LPS), concanavalin A (Con A), or phythaemagglutinin (PHA). Elevated levels of interleukin 6 (IL-6) and soluble IL-2 receptor (sIL-2R) were found 1 h after the run in both serum and urine samples. TNF-alpha in serum was also increased, whereas IL-2 in urine was decreased after the exercise. All other testings in serum and urine (including IFN-gamma) gave borderline or negative results. In cell cultures, the LPS-induced release of the inflammatory cytokines TNF-alpha, IL-1, and IL-6 was suppressed 1 h after exercise. Also, the Con-A-induced and LPS-induced release of IFN-gamma, and the PHA-induced release of IL-2 were suppressed 1 h after exercise. In contrast, Con-A-induced release of IL-2 was mildly increased after the run. We conclude that exercise of the intensity and duration described here causes an activation of the immune system, which is immediately counter-regulated. Twenty hours after the exercise, most of the observed changes were back to pre-exercise levels, indicating only a short duration for this suppressive counter-regulation.