ANALYSIS OF A GENOMIC DNA SEGMENT CARRYING THE WHEAT HIGH-MOLECULAR-WEIGHT (HMW) GLUTENIN BX17 SUBUNIT AND ITS USE AS AN RFLP MARKER
ANALYSIS OF A GENOMIC DNA SEGMENT CARRYING THE WHEAT HIGH-MOLECULAR-WEIGHT (HMW) GLUTENIN BX17 SUBUNIT AND ITS USE AS AN RFLP MARKER
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DOI：
10.1007/bf00220921
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发表时间：
1993-01-01
影响因子：
5.4
通讯作者：
APPELS, R
中科院分区：
文献类型：
--
作者：
REDDY, P;APPELS, R
从小麦基因组文库中克隆到一个含有Bx17高分子量麦谷蛋白基因的基因组片段。该片段含有2.82 kb的编码区，1.98 kb的下游侧翼区和12.8 kb的上游侧翼区。对该片段进行了测序，并与先前发表的1A、1B和1D染色体上的谷蛋白基因进行了比较。Bx17基因显示出与Bx7基因序列的显著相似性。一个表型树来自对齐。还显示了限制性片段长度多态性（RFLP）在麦谷蛋白基因座在一组澳大利亚和国际小麦品种使用不同区域的麦谷蛋白克隆作为探针。在所有品种的蛋白质组成的RFLPs相关性很好。
A genomic fragment containing the Bx17 high-molecular-weight (HMW) glutenin gene was isolated from a wheat genomic library. The fragment contains a coding region of 2.82 kb with 1.98-kb downstream and 12.8-kb upstream flanking regions. The fragment was sequenced and compared with previously published glutenin genes from chromosomes 1A, 1B and 1D using a computer alignment package. The Bx17 gene shows marked similarity to the Bx7 gene sequence. A phenetic tree derived from the alignments is presented. Also shown are restriction fragment length polymorphisms (RFLPs) at the glutenin loci in a set of Australian and international wheat varieties using different regions of the glutenin clone as probes. The RFLPs correlated well with the protein composition in all cultivars analysed.