Comparative genomic hybridization analysis detects frequent over-representation of DNA sequences at 3q, 7p, and 8q in head and neck carcinomas
Comparative genomic hybridization analysis detects frequent over-representation of DNA sequences at 3q, 7p, and 8q in head and neck carcinomas
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DOI：
10.1016/s0165-4608(99)00213-7
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发表时间：
2000-05-01
影响因子：
--
通讯作者：
Squire, JA
中科院分区：
文献类型：
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作者：
Bergamo, NA;Rogatto, SR;Squire, JA
应用比较基因组杂交（CGH）技术对19例头颈部鳞状细胞癌（HNSCC）进行染色体不平衡检测。染色体臂最常见或最不常见的是3q（48%），8 q（42%）和7 p（32%）;在许多情况下，这些变化在高拷贝数时观察到。其他常见的过度代表位点是1 q，2 q，6p，6 q和18 q。最常见的代表性不足的细分市场是3 p和22 q。在两个舌肿瘤中观察到22号染色体两个多态性微卫星位点的杂合性丢失，与CGH分析一致。在表现出复发和/或转移临床病史的患者中检测到1 q和2 q物质的增加，随后是终末期疾病。这种相关性提示1 q和2 q图谱的获得是头颈部肿瘤难治性临床反应的一个新标志。(C)2000 Elsevier Science Inc. All rights reserved.
Comparative genomic hybridization (CGH) was used to identify chromosomal imbalances in 19 samples of squamous cell carcinoma of the head and neck (HNSCC). The chromosome arms most often or er-represented were 3q (48%), 8q (42%), and 7p (32%); in many cases, these changes were observed at high copy number. Other commonly over-represented sites were 1q, 2q, 6p, 6q, and 18q. The most frequently under-represented segments were 3p and 22q. Loss of heterozygosity of two polymorphic microsatellite loci from chromosome 22 was observed in two tongue tumors, in agreement with the CGH analysis. Gains of 1q and 2q material were detected in patients exhibiting a clinical history of recurrence and/or metastasis followed by terminal disease. This association suggests that gain of 1q and 2q map be a new marker of head and neck tumors with a refractory clinical response. (C) 2000 Elsevier Science Inc. All rights reserved.