Effects of SIVmac infection on peripheral blood CD4+CD8+ T lymphocytes in cynomolgus macaques
Effects of SIVmac infection on peripheral blood CD4+CD8+ T lymphocytes in cynomolgus macaques
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DOI：
10.1006/clim.1999.4700
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发表时间：
1999-06-01
影响因子：
8.6
通讯作者：
Yoshikawa, Y
中科院分区：
文献类型：
--
作者：
Akari, H;Nam, KH;Yoshikawa, Y
我们之前报道过，具有静息记忆表型的CD4(+)CD8(+)双阳性(DP)T细胞存在于成年食蟹猴外周血淋巴细胞中相当大的比例。在这项研究中，我们检测了猕猴猿免疫缺陷病毒 (SIVmac) 感染对 DP T 细胞的影响。在体外，SIVmac239 nef-open (239) 及其 nef 缺失突变体在 CD4(+)CD8(-) 和 DP T 细胞中复制良好。然而，当猕猴感染239、DP而不是CD4(+)CD8(-)时，T细胞在细胞激活和病毒复制的同时短暂增加，随后在感染后1个月内耗尽。有趣的是，nef 基因是 DP T 细胞消耗所必需的，但不是增加和激活 DP T 细胞所必需的。这些数据表明，致病性 SN 感染可能通过与 CD4(+)CD8(-) T 细胞耗竭不同的 Nef 功能相关机制下调 DP T 细胞的产生和/或血液循环，(C) 1999 年学术出版社。
We have previously reported that CD4(+)CD8(+) double-positive (DP) T cells with a resting memory phenotype exist in a substantial proportion of peripheral blood lymphocytes of adult cynomolgus macaques. In this study, we examined the effects of simian immunodeficiency virus of macaque (SIVmac) infection on DP T cells. In vitro, SIVmac239 nef-open (239) and its nef-deletion mutant replicated well in both CD4(+)CD8(-) and DP T cells. However, when the macaques were infected with 239, DP, but not CD4(+)CD8(-), T cells were transiently increased in parallel with cell activation and viral replication, followed by depletion within 1 month postinfection. Interestingly, the nef gene was required for depletion but not for the increase and activation of DP T cells. These data suggest that the pathogenic SN infection may downmodulate production and/or blood circulation of DP T cells by a Nef function-related mechanism(s) different from that for the depletion of CD4(+)CD8(-) T cells, (C) 1999 Academic Press.