Post-termination complex disassembly by ribosome recycling factor, a functional tRNA mimic
Post-termination complex disassembly by ribosome recycling factor, a functional tRNA mimic
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DOI：
10.1093/emboj/21.9.2272
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发表时间：
2002-05-01
期刊：
影响因子：
11.4
通讯作者：
Kaji, A
中科院分区：
文献类型：
--
作者：
Hirokawa, G;Kiel, MC;Kaji, A
核糖体再循环因子（RRF）与延伸因子G（EF-G）一起分解终止后核糖体复合物。易位抑制剂，硫链丝菌素，紫霉素和氨基糖苷类，抑制tRNA和mRNA的释放后终止复合物。相反，夫西地酸和GTP类似物将EF-G固定在核糖体上，允许一轮tRNA易位，抑制mRNA而不是tRNA从复合物中释放。tRNA的释放是mRNA释放的先决条件，但仅在EF-G时部分发生。这些数据与RRF与A位点结合并易位至P位点，从P和E位点释放脱酰化tRNA的观点一致。最后一步，mRNA的释放，伴随着RRF和EF-G从核糖体的释放。使用模型终止后复合物，通过RRF反应从终止后复合物释放70 S核糖体，然后通过IF 3解离成亚基。
Ribosome recycling factor (RRF) together with elongation factor G (EF-G) disassembles the post- termination ribosomal complex. Inhibitors of translocation, thiostrepton, viomycin and aminoglycosides, inhibited the release of tRNA and mRNA from the post-termination complex. In contrast, fusidic acid and a GTP analog that fix EF-G to the ribosome, allowing one round of tRNA translocation, inhibited mRNA but not tRNA release from the complex. The release of tRNA is a prerequisite for mRNA release but partially takes place with EF-G alone. The data are consistent with the notion that RRF binds to the A-site and is translocated to the P-site, releasing deacylated tRNA from the P- and E-sites. The final step, the release of mRNA, is accompanied by the release of RRF and EF-G from the ribosome. With the model post-termination complex, 70S ribosomes were released from the post-termination complex by the RRF reaction and were then dissociated into subunits by IF3.