Hydroxyapatite-pulp composite fiber sheet bed: A new material for the immobilization of CHO-K1 cells
Hydroxyapatite-pulp composite fiber sheet bed: A new material for the immobilization of CHO-K1 cells
复制标题
羟基磷灰石-浆粕复合纤维片床：一种固定CHO-K1细胞的新材料
DOI：
10.1023/a:1008144305744
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发表时间：
2004
期刊：
影响因子：
2.2
通讯作者：
S. Shirahata
中科院分区：
文献类型：
--
作者：
Hironobu Kawakatsú;S. Ide;Y. Okuda;S. Shirahata
采用一种新型细胞培养固定材料--羟基磷灰石纸浆复合纤维（HAPC）片床来培养CHO-K1细胞。以HAPC纤维为原料，采用造纸工艺制备了细胞培养用的片状床。扫描电镜分析表明，HAPC板层由直径为10-200 μm的纤维堆积而成，孔表面积为0.32 m2 g-1。用25 × 25 mm ~ 2、0.41 mm厚（85 g m ~（-2）基重）的HAPC板床培养CHO-K1细胞，细胞密度达3.7 × 107个/cm ~（-3）。使用HAPC片床在500 ml转瓶中成功地进行了CHO-K1细胞的高密度培养，培养时间为21天。围绕搅拌桨缠绕的HAPC片床在旋转瓶中旋转，以有效地提供营养物并去除废物。HAPC板床具有大的表面积以支持细胞生长，并且在床内具有大的扩散空间。这种用于贴壁依赖性细胞的可降解基质可以容易地按比例放大。
A new immobilization material for cell culture, ahydroxyapatite-pulp composite fiber (HAPC) sheet bed, was usedto grow CHO-K1 cells. The sheet bed for cell culture wasprepared from HAPC fiber by paper-making techniques. Scanning electron microscopic analysis revealed that the HAPCsheet bed had a structure consisting of piled fibers with spaces 10–200 μm in diameter and a pore surface area of 0.32 m2g-1. Using a 25 × 25 mm2squareHAPC sheet bed 0.41 mm in thickness (85 g m-2basis weight) for cell culture, CHO-K1 cells grew to a cell densityof 3.7 × 107cells cm-3in a 60 mm plastic dish over a 6-day culture period. High-density culture of CHO-K1 cells was successfully performed using the HAPC sheet bed in a 500 ml spinner flask over a 21-day culture period. The HAPC sheet bed, wound around the stirrer paddle, was rotated in the spinner flask in order to supply nutrientsand remove waste products efficiently. The HAPC sheet bedhas a large surface area to support cell growth and there islarge diffusion space inside of the bed. This newautoclavable substrate for anchorage-dependent cells can be easily scaled-up.