Hard tissue regeneration capacity of apical pulp derived cells (APDCs) from human tooth with immature apex
Hard tissue regeneration capacity of apical pulp derived cells (APDCs) from human tooth with immature apex
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DOI：
10.1016/j.bbrc.2008.04.016
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发表时间：
2008-06-20
影响因子：
3.1
通讯作者：
Amagasa, Teruo
中科院分区：
文献类型：
--
作者：
Abe, Shigehiro;Yamaguchi, Satoshi;Amagasa, Teruo
最近的研究表明，牙髓是成体干细胞的新来源。具有未成熟根尖的人类牙齿是发育中的器官，并且该牙齿的根尖牙髓可能含有多种参与牙根形成的祖细胞/干细胞。我们研究了人根尖牙髓衍生细胞（APDC）的硬组织再生潜能。用矿化促进培养基培养的APDCs在多孔羟基磷灰石（HA）支架中显示出碱性磷酸酶活性。将APDCs和HA复合物植入免疫功能低下大鼠皮下，并在植入后12周收获。在组织学分析中，APDCs/HA复合材料在HA的孔隙区域显示出骨和牙本质样矿化组织。这项研究表明，人类牙齿与未成熟的根尖是一个有效的来源细胞的硬组织再生。(c)2008年爱思唯尔公司All rights reserved.
Recent studies indicate that dental pulp is a new source of adult stem cells. The human tooth with an immature apex is a developing organ, and the apical pulp of this tooth may contain a variety of progenitor/stem cells, which participate in root formation. We investigated the hard tissue regeneration potential of apical pulp derived cells (APDCs) from human tooth with an immature apex. APDCs cultured with a mineralization-promoting medium showed alkaline phosphatase activity in porous hydroxyapatite (HA) scaffolds. The composites of APDCs and HA were implanted subcutaneously in immunocompromised rats and harvested at 12 weeks after implantation. In histological analysis, the APDCs/HA composites exhibited bone- and dentine-like mineralized tissues in the pore areas of HA. This study suggests that the human tooth with an immature apex is an effective source of cells for hard tissue regeneration. (c) 2008 Elsevier Inc. All rights reserved.