Candida albicans double-stranded DNA can participate in the host defense against disseminated candidiasis
Candida albicans double-stranded DNA can participate in the host defense against disseminated candidiasis
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DOI：
10.1016/j.micinf.2004.10.011
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发表时间：
2005-02-01
影响因子：
5.8
通讯作者：
Ivanovska, N
中科院分区：
文献类型：
--
作者：
Yordanov, M;Dimitrova, P;Ivanovska, N
本文研究了双链白念珠菌DNA的体外免疫调节特性及其对小鼠播散性念珠菌病的保护作用。DNA通过氯喹依赖性机制诱导体外腹腔巨噬细胞和脾细胞产生TNF-α。酵母DNA与IFN-γ协同作用，触发巨噬细胞分泌一氧化氮，并使它们能够刺激T细胞增殖，以响应可溶性抗CD 3。DNA对脾细胞的作用与IFN-γ、IL-2和IL-10的合成增强有关。在体内，DNA可降低腹腔接种C.白念珠菌同时增加特异性增殖反应和细胞因子的产生。结果表明，C.白念珠菌DNA可以提供针对播散性感染的保护。(c)2005年，Elsevier SAS。All rights reserved.
In the present work, we studied the in vitro immunomodulatory properties of double-stranded Candida albicans DNA and its protective effect in murine disseminated candidiasis. DNA induced the production of TNF-alpha by peritoneal macrophages and splenocytes in vitro through a chloroquine-dependent mechanism. Yeast DNA acted synergistically with IFN-gamma in triggering the secretion of nitric oxide by macrophages and enabled them to stimulate the proliferation of T cells in response to soluble anti-CD3. The effect of DNA on splenocytes is associated with an enhanced synthesis of IFN-gamma, IL-2 and IL-10. In vivo, DNA decreased the mortality and lowered the kidney contamination in mice intraperitoneally inoculated with C. albicans simultaneously with an increase in the specific proliferative response and cytokine production. The present results indicate that C. albicans DNA can provide protection against disseminated infection. (c) 2005 Elsevier SAS. All rights reserved.