Cell recovery kinetics for split-dose, multifractionated and continuous irradiation in the DSB model.
Cell recovery kinetics for split-dose, multifractionated and continuous irradiation in the DSB model.
复制标题
DSB 模型中分次剂量、多分割和连续照射的细胞恢复动力学。
DOI：
10.1080/09553009314550071
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发表时间：
1993
影响因子：
2.6
通讯作者：
Ostashevsky,JY
中科院分区：
文献类型：
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作者：
Ostashevsky,JY
在DSB模型（Ostashevsky, 1989）的框架中考虑了分剂量、多次分割和连续照射的恢复动力学，并假设DSB是一种合作类型的修复。根据细胞镀的时间可以区分两种类型的体外分裂剂量实验：(i) IP，细胞在第二次剂量后立即镀；（ii） DP，即细胞在第二次给药后长时间孵育后再镀。该模型预测，IP分剂量恢复动力学主要依赖于第一次剂量，而不是由单一指数曲线描述。这些恢复动力学的速度比DSB修复的速度快。相比之下，DP分剂量恢复动力学与剂量无关，由单指数曲线描述，其时间常数与DSB修复的时间常数一致。在这项工作中得出的多次和连续照射后的细胞存活方程与其他模型（如不完全修复（IR））中的细胞存活方程不同，代表了一种值得测试的替代方案。根据这些方程估计的修复时间常数值比应用相同实验数据的红外方程估计的修复时间常数值至少长1.5 - 2倍。
The recovery kinetics for split-dose, multifractionated and continuous irradiations were considered in the framework of the DSB model (Ostashevsky 1989) with the assumption of a cooperative type of DSB repair. Two types ofin vitrosplit-dose experiments can be distinguished on the basis of the time of cell plating: (i) IP, where cells are plated immediately after the second dose; and (ii) DP, where cells are incubated for a long period after the second dose before plating. The model predicts that the IP split-dose recovery kinetics depend mainly on the first dose and are not described by a single exponential curve. The rate of these recovery kinetics is faster than that of DSB repair. In contrast, the DP split-dose recovery kinetics are dose-independent and described by a single exponential curve, the time constant of which coincides with that for DSB repair.The equation for cell survival after multifractionated and continuous irradiations, derived in this work, are different from those in other models (e.g. incomplete repair (IR)) and represent an alternative which is worth testing. The values of the repair time constant estimated from these equations are at least 1·5–2-fold longer than those estimated from the IR equations applied to the same experimental data.