The pathogenicity and host immune response associated with H5N1 highly pathogenic avian influenza virus in quail.
The pathogenicity and host immune response associated with H5N1 highly pathogenic avian influenza virus in quail.
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DOI：
10.1292/jvms.12-0433
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发表时间：
2013-05
期刊：
影响因子：
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通讯作者：
Yukiko Uno;T. Usui;Kosuke Soda;Y. Fujimoto;T. Takeuchi;Hiroshi Ito;Toshihiro Ito;Tsuyoshi Yamaguchi-Tsuyoshi-Yamaguc
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文献类型：
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作者：
Yukiko Uno;T. Usui;Kosuke Soda;Y. Fujimoto;T. Takeuchi;Hiroshi Ito;Toshihiro Ito;Tsuyoshi Yamaguchi-Tsuyoshi-Yamaguc
鹌鹑和鸡一样，对H5N1亚型高致病性禽流感病毒（HPAIV）敏感。这两种鸟的死亡率都很高，但鹌鹑通常比鸡多存活几天。为了了解原因，我们在接种 10(6) 50% 鸡蛋感染剂量的 HPAIV A/大天鹅/青森/1/2008 (H5N1) 后对鹌鹑和鸡进行了监测。鹌鹑接种后 48 小时 (h.p.i.) 和接种后 36 小时 (h.p.i.) 的临床过程开始为抑郁症。在鸡中，所有受感染的鸡都死亡。鹌鹑的平均死亡时间（91 小时）明显长于鸡的平均死亡时间（66 小时）。死前收集的大多数组织样本的病毒滴度没有显着差异。 24小时后，鹌鹑外周血单核细胞（PBMC）中的干扰素γ（IFN-γ）mRNA表达上调，但在鸡中下调，尽管TLR-7和其他七种细胞因子在鹌鹑和鸡之间没有显着差异。鹌鹑PBMC中的病毒载量显着低于鸡。这些结果表明鹌鹑感染 HPAIV 后诱导产生 IFN-γ 与 HPAIV 滴度降低有关。总之，感染 H5N1 HPAIV 的鹌鹑和鸡之间观察到的不同临床病程可能是由于针对 HPAIV 感染的 IFN-γ 反应不同所致。
Quail, like chickens, are susceptible to H5N1 subtype highly pathogenic avian influenza virus (HPAIV). Both birds experience high mortality, but quail usually survive a few more days than chicken. To understand why, we monitored quail and chickens after inoculation with 10(6) fifty-percent egg infectious doses of HPAIV A/whooper swan/Aomori/1/2008 (H5N1). The clinical course initiated as depression at 48 hr post inoculation (h.p.i.) in quail and at 36 h.p.i. in chicken, and all infected birds died. Mean death time of quail (91 hr) was significantly longer than that of chicken (66 hr). The virus titers of most tissue samples collected before death were not significantly different. At 24 h.p.i., interferon gamma (IFN-γ) mRNA expression in peripheral blood mononuclear cells (PBMC) was up-regulated in the quail but down-regulated in the chicken, although TLR-7 and seven other cytokines showed no significant differences between quail and chicken. The viral load in quail PBMC was significantly lower than that in chickens. These results suggest that the induction of IFN-γ after HPAIV infection in quail is related to lower titer of HPAIV. In conclusion, the different clinical courses observed between quail and chicken infected with H5N1 HPAIV might be caused by different IFN-γ responses against the HPAIV infection.