A bacterial gene, fip, required for filamentous bacteriophage fl assembly
A bacterial gene, fip, required for filamentous bacteriophage fl assembly
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丝状噬菌体 fl 组装所需的细菌基因 fip
DOI：
10.1128/jb.154.3.1064-1076.1983
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发表时间：
1983
影响因子：
3.2
通讯作者：
P. Model
中科院分区：
文献类型：
--
作者：
M. Russel;P. Model
不支持丝状噬菌体生长的大肠杆菌突变体允许噬菌体f1在突变细胞中合成DNA和表达基因，但后代颗粒不能组装。突变细胞没有其他明显的表型。通过对含有非致命性基因I突变的噬菌体进行的实验，以及选择能够在突变细菌上生长的突变体f1，我们提出了噬菌体基因i编码的形态发生功能与该突变体所改变的细菌功能之间的相互作用。细菌突变定义了一个新的基因，FIP(用于丝状噬菌体生产)，位于大肠杆菌染色体上84.2分钟附近。
An Escherichia coli mutant which does not support the growth of filamentous bacteriophage fl allows phage fl DNA synthesis and gene expression in mutant cells, but progeny particles are not assembled. The mutant cells have no other obvious phenotype. On the basis of experiments with phage containing nonlethal gene I mutations and with mutant fl selected for the ability to grow on mutant bacteria, we propose an interaction between the morphogenetic function encoded by gene I of the phage and the bacterial function altered in this mutant. The bacterial mutation defines a new gene, fip (for filamentous phage production), located near 84.2 min on the E coli chromosome.