Theoretical Analysis of Novel Quasi-3D Microscopy of Cell Deformation.
Theoretical Analysis of Novel Quasi-3D Microscopy of Cell Deformation.
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DOI：
10.1007/s12195-011-0218-3
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发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Guo, X. Edward
中科院分区：
文献类型：
--
作者：
Qiu, Jun;Baik, Andrew D.;Lu, X. Lucas;Hillman, Elizabeth M. C.;Zhuang, Zhuo;Dong, Cheng;Guo, X. Edward
发展了一种新的准三维(准3D)显微技术，可以同时在两个垂直平面上显示动态加载下的细胞。细胞在流体流动下的力学行为的三维(3D)动力学可以在高时间分辨率下被检测到。在本研究中，建立了荧光染色细胞的三维数值模型，通过有限元分析(FEA)对细胞进行单向变形或单向流体剪切流动。因此，准确地规定了模拟细胞内的变形。使用点扩展函数(PSF)和卷积运算，从细胞及其在3D空间中的变形状态生成模拟准3D技术的二维荧光图像。这些模拟的原始和变形的图像被数字图像相关技术处理以计算基于准3D的细胞内应变。计算的应变与规定的应变进行了比较，从而为基于准3D和广场显微镜的细胞内应变测量相对于真实3D应变的准确性提供了理论基础。模拟的准3D图像的信噪比(SNR)也使用加性高斯噪声进行调制，使用数字图像相关恢复指定应变所需的最小SNR为12。我们的计算研究表明，准三维应变测量接近地恢复了均匀和流体流动细胞应变状态下的真实三维应变，其应变误差在5%以内。
A novel quasi-three-dimensional (quasi-3D) microscopy technique has been developed to enable visualization of a cell under dynamic loading in two orthogonal planes simultaneously. The three-dimensional (3D) dynamics of the mechanical behavior of a cell under fluid flow can be examined at a high temporal resolution. In this study, a numerical model of a fluorescently dyed cell was created in 3D space, and the cell was subjected to uniaxial deformation or unidirectional fluid shear flow via finite element analysis (FEA). Therefore, the intracellular deformation in the simulated cells was exactly prescribed. Two-dimensional fluorescent images simulating the quasi-3D technique were created from the cell and its deformed states in 3D space using a point-spread function (PSF) and a convolution operation. These simulated original and deformed images were processed by a digital image correlation technique to calculate quasi-3D-based intracellular strains. The calculated strains were compared to the prescribed strains, thus providing a theoretical basis for the measurement of the accuracy of quasi-3D and wide-field microscopy-based intracellular strain measurements against the true 3D strains. The signal-to-noise ratio (SNR) of the simulated quasi-3D images was also modulated using additive Gaussian noise, and a minimum SNR of 12 was needed to recover the prescribed strains using digital image correlation. Our computational study demonstrated that quasi-3D strain measurements closely recovered the true 3D strains in uniform and fluid flow cellular strain states to within 5% strain error.
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影响因子：
1.3
作者：
Pan, Bing;Xie, Huimin;Hua, Tao
通讯作者：
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影响因子：
3.8
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DOI：
10.1016/j.bbrc.2010.02.115
发表时间：
2010-03-26
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作者：
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