Directional migration of MDA-MB-231 cells under oxygen concentration gradients.
Directional migration of MDA-MB-231 cells under oxygen concentration gradients.
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MDA-MB-231 细胞在氧浓度梯度下的定向迁移。
DOI：
10.1007/978-3-319-38810-6_17
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Takahashi E
中科院分区：
文献类型：
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作者：
Yahara D;Yoshida T;Enokida Y;Takahashi E
为了阐明癌细胞血行转移的初始机制，我们假设癌细胞沿着氧浓度梯度向氧浓度较高的区域（例如瘤内微血管）迁移。为了在体外产生氧气浓度梯度，我们设计了间隙盖玻璃（GCG）。将 GCG 置于培养的 MDA-MB-231 细胞（一种转移性乳腺癌细胞系）上后，在微型培养箱中、3% 氧气下，跟踪 GCG 下单个细胞的迁移长达 12 小时。我们使用前向迁移指数（FMI）量化单个细胞的迁移。垂直于氧气梯度的细胞迁移在该方向上是随机的，而位于距离氧气入口 300、500、700 和 1500 μm 处的细胞的 FMI 为正值（p< 0.05），表明向氧气入口单向迁移。目前的结果与我们的假设一致，即 MDA-MB-231 细胞迁移到氧浓度较高的区域。
To elucidate the initial mechanism of hematogenous metastasis of cancer cells, we hypothesized that cancer cells migrate toward regions with higher oxygen concentration such as intratumor micro vessels along the oxygen concentration gradient. To produce gradients of oxygen concentrationin vitro, we devised the gap cover glass (GCG). After placing a GCG onto cultured MDA-MB-231 cells (a metastatic breast cancer cell line), the migration of individual cells under the GCG was tracked up to 12 h at 3 % oxygen in the micro incubator. We quantified the migration of individual cells using forward migration index (FMI). The cell migration perpendicular to the oxygen gradients was random in the direction whereas FMIs of the cell located at 300, 500, 700, and 1500 μm from the oxygen inlet were positive (p < 0.05) indicating a unidirectional migration toward the oxygen inlet. Present results are consistent with our hypothesis that MDA-MB-231 cells migrate toward regions with higher oxygen concentration.