CD133+ neural stem cells in the ependyma of mammalian postnatal forebrain
CD133+ neural stem cells in the ependyma of mammalian postnatal forebrain
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DOI：
10.1073/pnas.0710000105
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发表时间：
2008-01-22
影响因子：
11.1
通讯作者：
Sun, Yi E.
中科院分区：
文献类型：
--
作者：
Coskun, Volkan;Wu, Hao;Sun, Yi E.
出生后的前脑脑室下区（SVZ）藏有干细胞，这些干细胞在整个生命过程中产生嗅球中间神经元。成人SVZ中干细胞的身份已被广泛讨论。虽然室管膜细胞曾被认为具有干细胞特征，但随后的研究挑战了最初的报道，并假设室管膜下GFAP（+）细胞是干细胞。在这里，我们报告说，在成年小鼠前脑，免疫反应的神经干细胞标记物，CD 133 -1/CD 133，是专门定位于室管膜，虽然不是所有的室管膜细胞是CD 133（+）。利用移植和遗传谱系追踪的方法，我们证明了CD 133（+）室管膜细胞持续产生新的神经元，注定嗅球。总的来说，我们的数据表明，与表达GFAP的成体神经干细胞相比，CD 133（+）室管膜细胞代表了哺乳动物前脑中额外的-也许是更多的静止-干细胞群。
The postnatal forebrain subventricular zone (SVZ) harbors stem cells that give rise to olfactory bulb interneurons throughout life. The identity of stem cells in the adult SVZ has been extensively debated. Although, ependymal cells were once suggested to have stem cell characteristics, subsequent studies have challenged the initial report and postulated that subependymal GFAP(+) cells were the stem cells. Here, we report that, in the adult mouse forebrain, immunoreactivity for a neural stem cell marker, prominin-1/CD133, is exclusively localized to the ependyma, although not all ependymal cells are CD133(+). Using transplantation and genetic lineage tracing approaches, we demonstrate that CD133(+) ependymal cells continuously produce new neurons destined to olfactory bulb. Collectively, our data indicate that, compared with GFAP expressing adult neural stem cells, CD133(+) ependymal cells represent an additional-perhaps more quiescent-stem cell population in the mammalian forebrain.