Mapping of the phenol sulfotransferase gene (STP) to human chromosome 16p12.1-p11.2 and to mouse chromosome 7.
Mapping of the phenol sulfotransferase gene (STP) to human chromosome 16p12.1-p11.2 and to mouse chromosome 7.
复制标题
将苯酚磺基转移酶基因 (STP) 映射到人类染色体 16p12.1-p11.2 和小鼠染色体 7。
DOI：
10.1006/geno.1993.1494
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Siciliano,MJ
中科院分区：
文献类型：
--
作者：
Dooley,TP;Obermoeller,RD;Leiter,EH;Chapman,HD;Falany,CN;Deng,Z;Siciliano,MJ
我们最近克隆了一个cDNA编码人类酚偏好的苯酚磺酰基氨基转氨酶（P-PST）酶。基于humanSTP一对寡核苷酸引物(代表sulfotransferase phenol-preferring) cDNA序列合成,采用聚合酶链反应(PCR)扩增人类基因组DNA识别525 bp的DNA片段。确定了stpgene在编码区5 '端附近的这部分DNA序列。扩增的基因组片段包含两个小内含子，分别为104和89 bp。当使用这些引物对人类仓鼠体细胞杂交组的DNA样本进行PCR筛选时，只有那些包含人类16号染色体的杂交体对525-bp基因组片段呈阳性。为了确定16号染色体上含有stpgene的特定区域，在包含人类16号染色体特定部分的人-鼠体细胞杂交板上进行PCR扩增反应。结果表明，stpis定位在16p11.2至p12远端蛋白激酶C β1多聚体基因（PRKCB1）的近端。1. 人类stp基因定位于巴滕病（CLN3）位点附近。此外，我们通过对小鼠种间回交后代的基因分型确定，小鼠的同源基因（Stp）定位于小鼠7号染色体上靠近d7mit8（在54 cM处）和d7bir1标记的共合区域。
We have recently cloned a cDNA encoding the human phenol-preferring phenol sulfotranaferase (P-PST) enzyme. An oligonucleotide primer pair based on the humanSTP(representing sulfotransferase, phenol-preferring) cDNA sequence was synthesized and was employed in polymerase chain reaction (PCR) amplification of human genomic DNA to identify a 525-bp DNA fragment. The DNA sequence of this portion of theSTPgene, near the 5′ end of the coding region, was determined. The amplified genomic fragment contained two small introns of 104 and 89 bp. When DNA samples from a human-hamster somatic cell hybrid panel were screened by PCR using these primers, only those hybrids that contained human chromosome 16 were positive for the 525-bp genomic fragment. To identify the specific region on chromosome 16 that contained theSTPgene, PCR amplification reactions were performed on a human-mouse somatic cell hybrid panel containing defined portions of human chromosome 16. The results indicated thatSTPis localized proximal to the gene for protein kinase C, β1 polypepride (PRKCB1), in the region from the distal portion of 16p11.2 to p12. 1. The humanSTPgene maps near the locus for Batten disease (CLN3). Furthermore, we have determined by genotyping of murine interspecific backcross progeny that the homologous gene in mouse (Stp) localizes to the syntenic region of mouse chromosome 7 near theD7Mit8(at 54 cM) andD7Bir1markers.