The Role of the Substrate Lipid in Processive Glycan Polymerization by the Peptidoglycan Glycosyltransferases
The Role of the Substrate Lipid in Processive Glycan Polymerization by the Peptidoglycan Glycosyltransferases
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DOI：
10.1021/ja909325m
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发表时间：
2010-01-13
影响因子：
15
通讯作者：
Walker, Suzanne
中科院分区：
文献类型：
--
作者：
Perlstein, Deborah L.;Wang, Tsung-Shing Andrew;Walker, Suzanne
肽聚糖糖基转移酶 (PGT) 催化 C55 脂质连接二糖 (Lipid II) 的持续聚合，形成肽聚糖，这是细菌细胞壁的主要成分。由于在确定合适的底物类似物来选择性询问供体（延长链）和受体（脂质 II）位点方面存在挑战，我们理解该反应的能力受到限制。为了解决这个问题，我们开发了一种使用合成底物的检测方法，可以区分 PGT 的供体和受体位点。我们已经证明每个位点都有不同的脂质长度偏好。我们还确定，持续聚合取决于附着在供体上的脂质的长度。
The peptidoglycan glycosyltransferases (PGTs) catalyze the processive polymerization of a C55 lipid-linked disaccharide (Lipid II) to form peptidoglycan, the main component of the bacterial cell wall. Our ability to understand this reaction has been limited due to challenges identifying the appropriate substrate analogues to selectively interrogate the donor (the elongating strand) and acceptor (Lipid II) sites. To address this problem, we have developed an assay using synthetic Substrates that can discriminate between the donor and acceptor sites of the PGTs. We have shown that each site has a distinct lipid length preference. We have also established that processive polymerization depends on the length of the lipid attached to the donor.