Cellular mechanisms of Mullerian duct formation in the mouse
Cellular mechanisms of Mullerian duct formation in the mouse
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DOI：
10.1016/j.ydbio.2007.03.027
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发表时间：
2007-06-15
影响因子：
2.7
通讯作者：
Behringer, Richard R.
中科院分区：
文献类型：
--
作者：
Orvis, Grant D.;Behringer, Richard R.
不管它们的性染色体核型如何，黑猩猩都发育了两对生殖管，沃尔夫管和缪勒管。当苗勒管形成时，其生长尖端与沃尔夫管密切相关，因为它延伸到尿生殖窦。以往的研究表明，存在的Wolffian管的发展和维持的Mullerian管。苗勒管是由体腔上皮内陷形成的，但其延伸至尿生殖窦的机制尚不清楚。使用遗传命运映射，我们表明，沃尔夫管不贡献细胞的苗勒管。实验胚胎学操作和分子研究表明，前体细胞在尾尖的苗勒管增殖存款的细胞带沿着长度的泌尿生殖嵴。此外，免疫组织化学分析表明，发育中的苗勒管细胞沉积时是中上皮细胞，随后分化成上皮管，并最终分化成女性生殖道。我们的研究明确了苗勒管形成的细胞和分子机制。(C)2007年爱思唯尔公司All rights reserved.
Regardless of their sex chromosome karyotype, amniotes develop two pairs of genital ducts, the Wolffian and Mullerian ducts. As the Mullerian duct forms, its growing tip is intimately associated with the Wolffian duct as it elongates to the urogenital sinus. Previous studies have shown that the presence of the Wolffian duct is required for the development and maintenance of the Mullerian duct. The Mullerian duct is known to form by invagination of the coelomic epithelium, but the mechanism for its elongation to the urogenital sinus remains to be defined. Using genetic fate mapping, we demonstrate that the Wolffian duct does not contribute cells to the Mullerian duct. Experimental embryological manipulations and molecular studies show that precursor cells at the caudal tip of the Mullerian duct proliferate to deposit a cord of cells along the length of the urogenital ridge. Furthermore, immunohistochemical analysis reveals that the cells of the developing Mullerian duct are mesoepithelial when deposited, and subsequently differentiate into an epithelial tube and eventually the female reproductive tract. Our studies define cellular and molecular mechanisms for Mullerian duct formation. (C) 2007 Elsevier Inc. All rights reserved.