Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development.
Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development.
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DOI：
10.1101/pdb.prot5509
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发表时间：
2010-10-01
影响因子：
--
通讯作者：
Duman-Scheel, Molly
中科院分区：
文献类型：
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作者：
Haugen, Morgan;Tomchaney, Michael;Duman-Scheel, Molly
吸血蚊子，包括登革热和黄热病媒介埃及伊蚊，传播世界上许多最致命的疾病。这些疾病在发展中国家死灰复燃，对发达国家爆发流行病构成明显威胁。最近的蚊子基因组计划激发了人们对通过对媒介昆虫进行遗传操作来控制节肢动物传播疾病的潜力的兴趣，而调节发育的基因尤其令人感兴趣。该方法可用于分析伊蚊的基因表达。埃及伊蚊胚胎和幼虫，这是了解该媒介蚊子发育基因功能的关键方面。
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol for whole-mount in situ hybridization can be used to analyze gene expression in Ae. aegypti embryos and larvae, a critical aspect of understanding developmental gene function in this vector mosquito.