Mixtures of Tea andCitrus maxima(pomelo) Alleviate Lipid Deposition in HepG2 Cells Through the AMPK/ACC Signaling Pathway
Mixtures of Tea andCitrus maxima(pomelo) Alleviate Lipid Deposition in HepG2 Cells Through the AMPK/ACC Signaling Pathway
复制标题
茶和柚子的混合物通过 AMPK/ACC 信号通路减轻 HepG2 细胞中的脂质沉积
DOI：
10.1089/jmf.2020.4706
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发表时间：
2020-07-24
影响因子：
2.4
通讯作者：
Sun, Shi-Li
中科院分区：
文献类型：
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作者：
An, Ran;Wen, Shuai;Sun, Shi-Li
茶和柑橘是天然的药用同源植物，通常用于制作饮料，具有抗癌、抗肥胖和抗氧化特性。将绿茶、黄茶、红茶与柑橘组合得到绿茶与柑橘(GTCM)、黄茶与柑橘(YTCM)、红茶与柑橘(BTCM)。分析了这些混合物的生化成分，并探讨了它们在缓解肝脏脂质沉积方面的可能作用和机制。 YTCM和GTCM的茶多酚、游离氨基酸、酚胺比和咖啡因相当，显着高于BTCM。此外，YTCM中酯化儿茶素、非酯化儿茶素和总儿茶素的含量显着高于GTCM和BTCM。所有三种柑橘混合物均显着减少 HepG2 细胞中的脂质沉积，其中 GTCM 和 YTCM 比 BTCM 稍有效。对于可能的机制，Western blot分析显示，三种柑橘混合物可以激活AMPK/ACC信号通路，上调p-AMPK、p-ACC和CPT-1蛋白的表达，下调SREBP1c和脂肪酸合酶蛋白的表达，从而抑制脂肪合成，从而缓解肝细胞中的脂质沉积。总之，柑橘茶作为一种新型健康饮料，具有减轻肝脏脂质沉积的潜力，并进一步可能有助于肥胖的治疗。
Tea and citrus maxima are natural, medicinal homologous plants, typically used for making beverages, which have anticancer, antiobesity, and antioxidation properties. Green tea, yellow tea, and black tea were combined with citrus maxima to obtain green tea andCitrus maxima(GTCM), yellow tea andCitrus maxima(YTCM), and black tea andCitrus maxima(BTCM). The biochemical components of these mixtures were analyzed, and their possible effects and mechanisms on relieving liver lipid deposition were explored. The tea polyphenols, free amino acids, phenolamine ratio, and caffeine were comparable in YTCM and GTCM, being significantly higher than those in BTCM. In addition, the content of esterified catechins, nonesterified catechins, and total catechins in YTCM was significantly higher than those in GTCM and BTCM. All three mixtures ofCitrus maximatea significantly reduced lipid deposition in HepG2 cells, with GTCM and YTCM being slightly more effective than BTCM. Regarding the possible mechanism, Western blot analysis revealed that the threeCitrus maximatea mixtures could activate the AMPK/ACC signaling pathway, upregulate the expression of p-AMPK, p-ACC, and CPT-1 proteins, and downregulate the expression of SREBP1c and fatty acid synthase proteins to inhibit fat synthesis, thereby relieving lipid deposition in liver cells. In conclusion, as a novel and healthy beverage,Citrus maximatea has the potential to alleviate liver lipid deposition, and further could be responsible for obesity treatment.