Use of high-resolution confocal imaging of the vaginal epithelial microstructure to detect microbicide toxicity.
Use of high-resolution confocal imaging of the vaginal epithelial microstructure to detect microbicide toxicity.
复制标题
使用阴道上皮微观结构的高分辨率共聚焦成像来检测杀菌剂的毒性。
DOI：
10.1086/598221
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Bourne,Nigel
中科院分区：
文献类型：
--
作者：
Vargas,Gracie;Shilagard,Tuya;Johnston,Rebecca;Bell,Brent;Stegall,RachaelL;Vincent,Kathleen;Stanberry,Lawrence;Motamedi,Massoud;Bourne,Nigel
高分辨率光学成像共聚焦反射显微镜（CRM）的能力，描绘阴道上皮细胞的微观结构，并检测可能导致使用阴道杀微生物剂MethodsThe瑞士韦伯斯特小鼠醋酸甲羟孕酮治疗的阴道在体内暴露于4%壬苯醇醚-9（N-9）的凝胶或盐水。在治疗后4 h、16 h或48 h取出阴道，用CRM不染色成像，并从成像区域获得活检标本并进行组织学分析。结果在对照小鼠中，CRM显示柱状上皮和固有层的特征类似于通过组织学分析观察到的特征。CRM显示N-9处理后4 h和16 h上皮脱落，上皮厚度的定量测量显示对照样本的平均厚度（±平均值的标准误差）为41.7±1.7 μm，而处理后4 h获得的样本为14.9±4.5 μm，处理后16 h获得的样本为24.4±2.1 μm。治疗后4 h通过检测炎性浸润来指示炎症。在处理后48小时收集的样品中，上皮正在再生。结论CRM能较好地反映N-9治疗后阴道上皮细胞的形态结构和炎症反应，可作为评价阴道杀菌剂疗效的有效影像学工具
ObjectiveHigh-resolution optical imaging by confocal reflectance microscopy (CRM) was investigated for its ability to delineate the epithelial microstructure of the vaginal tract and detect alterations that may result from the use of vaginal microbicidesMethodsThe vaginal tracts of Swiss Webster mice treated with medroxyprogesterone acetate were exposed in vivo to a 4% nonoxynol-9 (N-9)–containing gel or saline. The vaginal tract was removed 4 h, 16 h, or 48 h after treatment and imaged by CRM without staining, and biopsy specimens were obtained from the imaged regions and processed for histological analysisResultsIn control mice, CRM revealed a columnar epithelium and lamina propria with features resembling those observed via histological analysis. CRM revealed an exfoliated epithelium 4 h and 16 h after N-9 treatment, and quantitative measurement of epithelial thickness revealed a mean thickness (± standard error of the mean) of ∼41.7±1.7 μm in control specimens, compared with 14.9±4.5 μm for specimens obtained 4 h after treatment and 24.4±2.1 μm for specimens obtained 16 h after treatment. Inflammation 4 h after treatment was indicated through detection of inflammatory infiltrates. In samples collected 48 h after treatment, the epithelium was regenerating. The time line of changes in the morphological structure and epithelial thickness detected by CRM closely resembled that of changes revealed by histological analysisConclusionsThis study demonstrates that CRM can delineate the epithelial structure and detect indicators of inflammation after treatment with N-9 and that it may be a useful imaging tool for evaluating the effects of vaginal microbicides
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