CircDHRS3 inhibits prostate cancer cell proliferation and metastasis through the circDHRS3/miR-421/MEIS2 axis.
CircDHRS3 inhibits prostate cancer cell proliferation and metastasis through the circDHRS3/miR-421/MEIS2 axis.
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DOI：
10.1080/15592294.2023.2178802
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发表时间：
2023-12
期刊：
影响因子：
3.7
通讯作者：
Jiang, Haowen
中科院分区：
文献类型：
--
作者：
Dai, Xiyu;Chen, Xinan;Chen, Wensun;Ou, Yuxi;Chen, Yiling;Wu, Siqi;Zhou, Quan;Yang, Chen;Zhang, Limin;Jiang, Haowen
前列腺癌是全世界男性中最常见的癌症类型。环状RNA(CircRNA)在前列腺癌中的重要性及其与恶性肿瘤的关系已被稳定地认识到。对前列腺癌的CircRNA进行测序，获得CircRNA的表达。进一步分析了CircRNA及其功能。用QRT-PCR、RIP、FISH、RNA Pull-down、WB、CCK-8、集落形成实验和伤口愈合实验对结果进行了验证。采用Balb/c裸鼠作为异种移植宿主。前列腺癌中CircDHRS3的低表达被评估。过表达CircDHRS3可抑制前列腺癌的体外生长和迁移。此外，荧光原位杂交和双荧光素酶实验表明，miR-421是CircDHRS3的下游靶标。PC3和Du145细胞的挽救实验结果表明，CircDHRS3通过CircDHRS3/miR-421/MEIS2轴调控MEIS2的表达，从而抑制前列腺癌细胞的增殖和转移能力。体内研究证实，CircDHRS3过表达对前列腺癌细胞的肺转移和骨转移均有抑制作用。CircDHRS3有可能成为前列腺癌的生物标记物和靶向治疗部位，特别是在前列腺癌的恶性阶段。我们的研究表明，CircDHRS3通过CircDHRS3/miR-421/MEIS2轴抑制前列腺癌细胞的增殖和转移。
Prostate cancer is the most prevalent type of cancer among men worldwide. The importance of circular RNA (circRNA) in prostate cancer and its connection to malignancy has been steadily recognized. circRNA expression was obtained by circRNA sequencing of prostate cancer. circRNA and its function were further analysed. The results were verified by qRT-PCR, RIP assay, FISH, RNA pulldown, WB, CCK-8, colony formation assay and wound-healing assay. BALB/c Nude mice were used for xenograft hosts. Low expression of circDHRS3 was assessed in prostate cancer. Overexpression of circDHRS3 inhibited prostate cancer growth and migration in vitro. Additionally, miR-421 was shown to be the downstream target of circDHRS3, as shown by fluorescence in situ hybridization and dual-luciferase experiments. The rescue assay results for the PC3 and Du145 cell lines demonstrated that circDHRS3 inhibits prostate cancer cell lines’ ability to proliferate and metastasize by modulating MEIS2 expression through the circDHRS3/miR-421/MEIS2 axis. In vivo investigations confirmed that the overexpression of circDHRS3 could inhibit both the lung and bone metastasis of prostate cancer cells. circDHRS3 has the potential to become a biomarker and a targeted therapeutic site for prostate cancer, particularly in the malignant stage. Our study indicates that circDHRS3 inhibits prostate cancer cell proliferation and metastasis through the circDHRS3/miR-421/MEIS2 axis.
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影响因子：
7
作者：
Ou Y;Dai X;Chen X;Chen Y;Wu S;Zhou Q;Yang C;Jiang H
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
16
作者：
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DOI：
10.1158/1078-0432.ccr-12-0373
发表时间：
2012-08-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
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通讯作者：
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