ANALYSIS OF CONTRACTILE PROPERTIES OF MUSCLES FROM RATS IMMUNIZED WITH PURIFIED ACETYLCHOLINE-RECEPTOR
ANALYSIS OF CONTRACTILE PROPERTIES OF MUSCLES FROM RATS IMMUNIZED WITH PURIFIED ACETYLCHOLINE-RECEPTOR
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DOI：
10.1016/0165-5728(87)90013-0
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发表时间：
1987-04-01
影响因子：
3.3
通讯作者：
KROLICK, KA
中科院分区：
文献类型：
--
作者：
OLSBERG, CA;MAXWELL, LC;KROLICK, KA
用纯化的乙酰胆碱受体（AChR）诱导大鼠实验性自身免疫性重症肌无力（EAMG）。除了检测血清中抗乙酰胆碱受体抗体滴度升高，我们观察到减少抽搐张力在次最大刺激电压和增加箭毒敏感性从免疫大鼠获得的肌肉相比，从nonimmune控制大鼠获得的肌肉。此外，抗体诱导的神经肌肉损伤的表达程度不同，这取决于是否检查了膈肌、比目鱼肌或趾长伸肌。因此，我们得出结论，潜在的抗体干扰AChR功能将不仅取决于抗体的性质，而且还存在于个别肌肉的复杂的结构-功能关系。这可能部分解释了肌无力患者不同肌群的不同损害。
Experimental autoimmune myasthenia gravis (EAMG) was induced in rats by injection of purified acetylcholine receptor (AChR). In addition to detecting elevated serum titers of anti-AChR antibodies, we observed decreased twitch-tension at submaximal stimulation voltages and increased curare sensitivity by muscles obtained from immunized rats when compared to muscles obtained from nonimmune control rats. Furthermore, antibody-induced neuromuscular impairment was expressed to differing extents dependent on whether the diaphragm, soleus, or extensor digitorum longus muscle was examined. Thus, we conclude that potential antibody perturbation of AChR function will depend not only on the nature of the antibody, but also on the complex structure-function relationships that exist in individual muscles. This may partially explain the variable impairment of different muscle groups in patients with myasthenia.