Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation
Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation
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DOI：
10.1242/dev.048405
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发表时间：
2010-10-15
期刊：
影响因子：
4.6
通讯作者：
Becker, Peter B.
中科院分区：
文献类型：
--
作者：
Chioda, Mariacristina;Vengadasalam, Sandra;Becker, Peter B.
果蝇核小体重构复合物CHRAC和ACF被认为在染色质组装和核小体动力学中起着全局性的作用。破坏编码共同的ACF 1亚基的基因会损害果蝇的生存能力。幸存者在所有发育阶段都表现出染色质组装和染色质介导的基因抑制缺陷。我们现在表明，ACF1的表达受到严格的发育控制。在胚胎发育过程中表达强烈减弱，仅在未分化的细胞中保持高水平，包括生殖细胞前体细胞和幼虫成神经细胞。ACF 1的组成型表达是致死的。细胞特异性异位表达扰乱染色质组织和核程序。通过监测异染色质的形成过程中的发展，我们发现，ACF 1包含的因素参与了最初建立多样化的染色质结构，如异染色质。改变ACF 1的水平导致全局和杂色偏离正常的染色质组织与多效性缺陷。
The nucleosome remodelling complexes CHRAC and ACF of Drosophila are thought to play global roles in chromatin assembly and nucleosome dynamics. Disruption of the gene encoding the common ACF1 subunit compromises fly viability. Survivors show defects in chromatin assembly and chromatin-mediated gene repression at all developmental stages. We now show that ACF1 expression is under strict developmental control. The expression is strongly diminished during embryonic development and persists at high levels only in undifferentiated cells, including the germ cell precursors and larval neuroblasts. Constitutive expression of ACF1 is lethal. Cell-specific ectopic expression perturbs chromatin organisation and nuclear programmes. By monitoring heterochromatin formation during development, we have found that ACF1-containing factors are involved in the initial establishment of diversified chromatin structures, such as heterochromatin. Altering the levels of ACF1 leads to global and variegated deviations from normal chromatin organisation with pleiotropic defects.