Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes
Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes
复制标题
通过蛋白质组的 iTRAQ 分析筛选双峰驼的生殖生物标志物
DOI：
10.1111/rda.13607
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发表时间：
2020-01-02
影响因子：
1.7
通讯作者：
Zhao, Xingxu
中科院分区：
文献类型：
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作者：
Wang, Qi;Zhang, Quanwei;Zhao, Xingxu
双峰驼是一种古老而珍贵的家畜，是沙漠中特有的资源，具有重要的经济和科学价值。近年来，双峰驼的数量急剧下降。由于其生殖周期长，发情季节性强，其发情机制尚不清楚。为了确定候选的生物标志物的繁殖，我们进行了全面的蛋白质组学分析的血清从双峰驼在发情和非发情，使用同量异位素标签的相对和绝对定量（iTRAQ）加上串联质谱。我们确定了359个蛋白质，其中32个差异表达：11个上调，21个在发情期骆驼样品中下调。我们使用qPCR和Western印迹验证了这些蛋白质的一个子集的差异表达。基因本体论注释发现差异表达蛋白在细胞过程、代谢过程和免疫系统过程中发挥作用。值得注意的是，五个差异表达的蛋白质，PCGF5，组蛋白H1.2，RBP4，FOLR1和ANTXR2，参与其他动物的生殖调控过程。KEGG富集分析表明，在几个心脏相关的途径，如“扩张性心肌病”，“肥厚性心肌病”，“心肌收缩”和“肾上腺素能信号传导心肌病”显着富集。我们的研究结果表明，候选生物标志物（PCGF5，组蛋白H1.2，RBP4，FOLR1和ANTXR2）的发现可以帮助理解双峰驼的繁殖。我们的结论是，分析血清蛋白质组，然后用更有针对性的方法测量选定的蛋白质，为研究发情机制提供了一种有前途的方法。
Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non-oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up-regulated and 21 were down-regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac-related pathways, such as 'dilated cardiomyopathy', 'hypertrophic cardiomyopathy', 'cardiac muscle contraction' and 'adrenergic signalling in cardiomyopathy'. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus.