Chemical Control over Cellular Uptake of Organic Nanoparticles by Fine Tuning Surface Functional Groups
Chemical Control over Cellular Uptake of Organic Nanoparticles by Fine Tuning Surface Functional Groups
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DOI：
10.1021/acsnano.5b03909
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发表时间：
2015-10-01
期刊：
影响因子：
17.1
通讯作者：
Zimmerman, Steven C.
中科院分区：
文献类型：
--
作者：
Bai, Yugang;Xing, Hang;Zimmerman, Steven C.
已知在纳米颗粒表面（NP）表面显示的功能组在NP细胞摄取中起重要作用。但是，仅报告了少数系统的研究来解决它们的作用，这在很大程度上是因为难以定期改变NP表面官能团的数量和结构。我们采用自下而上的策略来合成具有不同大小和功能组的水溶性有机纳米颗粒（ONPS），并使用易于使用的单体。利用流式细胞仪，我们测量了含有不同（a）长度，（b）羟基数量的侧链的ONP的HeLa细胞摄取效率，以及（c）甲基数。我们还研究了具有相同官能团但大小不同的ONP。使用相同的方法，但在血清存在下检查了蛋白电晕的潜在形成和影响。结果表明，在血清和无血清条件下，表面暴露的官能团决定了细胞摄取颗粒的效率，并且可以通过聚合物ONP重复单元的亲脂性来部分预测趋势。同样，通过使用“掩盖”策略，这些颗粒的细胞摄取行为可以方便地改变。
The functional groups displayed on the surface of nanoparticles (NP) are known to play an important role in NP cellular uptake. However, only a few systematic studies have been reported to address their role, in large part because of the difficulty in regularly varying the number and structure of the functional groups on the NP surface. We employ a bottom-up strategy for the synthesis of water-soluble organic nanoparticles (ONPs) with different sizes and functional groups, using readily available monomers. Utilizing flow cytometry, we measured the HeLa cell uptake efficiency of ONPs that contain side-chains with a different (a) length, (b) number of hydroxyl groups, and (c) number of methyl groups. We have also investigated ONPs with the same functional groups but different sizes. The potential formation and influence of protein corona was examined using the same approach but in the presence of serum. The results demonstrate that under both serum and serum-free conditions the surfaceexposed functional groups determine the efficiency of cellular uptake of the particles, and that the trend can be partially predicted by the lipophilicity of the polymeric ONP's repeating units. Also, by using a "masking" strategy, these particles' cellular uptake behavior could be altered conveniently.