LACTOSE GENES FUSED TO EXOGENOUS PROMOTERS IN ONE-STEP USING A MU-LAC BACTERIOPHAGE - INVIVO PROBE FOR TRANSCRIPTIONAL CONTROL SEQUENCES
LACTOSE GENES FUSED TO EXOGENOUS PROMOTERS IN ONE-STEP USING A MU-LAC BACTERIOPHAGE - INVIVO PROBE FOR TRANSCRIPTIONAL CONTROL SEQUENCES
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DOI：
10.1073/pnas.76.9.4530
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
COHEN, SN
中科院分区：
文献类型：
--
作者：
CASADABAN, MJ;COHEN, SN
将乳糖结构基因（不含乳糖启动子）整合到噬菌体Mu基因组中以形成Mu-lac特异性转导噬菌体。该噬菌体还携带编码氨苄青霉素抗性（Ap）的基因[Mu（Ap，lac）]。在感染后和建立溶原性后，Mu（Ap，lac）基因组可以整合到大肠杆菌染色体中明显随机的位点。当整合发生在基因内的转录方向时，乳糖结构基因的位置使得它们仅从该基因的启动子表达。因此，Mu（Ap，lac）的乳糖基因的表达可用作该基因转录的测定以及基因调控的功能和突变研究。
The lactose structural genes, without the lactose promoter, were incorporated into the bacteriophage Mu genome to form a Mu-lac specialized transducing phage. This phage also carries a gene encoding resistance to ampicillin (Ap) [Mu(Ap, lac)]. After infection and upon establishment of lysogeny, the Mu(Ap, lac) genome can integrate into apparently random sites in the Escherichia coli chromosome. When integration occurs within a gene in the orientation of its transcription, the lactose structural genes are so situated that they become expressed solely from the promoter of that gene. Thus, expression of the lactose genes of Mu(Ap, lac) can be used as an assay for transcription of that gene and for functional and mutational studies of gene regulation.