Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase
Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase
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DOI：
10.1007/s10529-011-0576-x
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发表时间：
2011-07-01
影响因子：
2.7
通讯作者：
Kenji, Kida
中科院分区：
文献类型：
--
作者：
An, Ming-Zhe;Tang, Yue-Qin;Kenji, Kida
在30℃和38℃条件下，研究了海藻糖-6-磷酸(T6P)合成酶基因(TPS1)的过表达对酿酒酵母乙醇发酵的影响。TPS1过表达显著提高了酒精发酵过程中T6P合酶的活性和海藻糖的积累，尤其是在38A℃时，TPS1基因过表达的转化子产生的乙醇约为38A℃。分别为60和37g/L。TPS1基因过表达的转化子在38A℃和30A℃下的发酵效率相似，临界生长温度从36℃提高到42A℃。这些结果表明，TPS1基因的过表达对TITLE酵母菌株在高温下的发酵能力有有利的影响。
The effect of overexpression of the trehalose-6-phosphate (T6P) synthase gene (TPS1) on ethanol fermentation of Saccharomyces cerevisiae has been studied at 30 and 38A degrees C. The activity of T6P synthase and the accumulation of trehalose during ethanol fermentation were significantly improved by overexpression of TPS1, and especially at 38A degrees C. Ethanol produced by transformants with and without TPS1 gene overexpression at 38A degrees C was approx. 60 and 37 g/l, respectively. The fermentation efficiency of transformants with TPS1 gene overexpression at 38A degrees C was similar to that at 30A degrees C. The critical growth temperature was increased from 36 to 42A degrees C by TPS1 gene overexpression. These results indicated that overexpression of the TPS1 gene had a beneficial effect on the fermentation capacity of the title yeast strain at high temperatures.