The herpesvirus saimiri ORF50 gene, encoding a transcriptional activator homologous to the Epstein-Barr virus R protein, is transcribed from two distinct promoters of different temporal phases
The herpesvirus saimiri ORF50 gene, encoding a transcriptional activator homologous to the Epstein-Barr virus R protein, is transcribed from two distinct promoters of different temporal phases
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疱疹病毒 saimiri ORF50 基因编码与 Epstein-Barr 病毒 R 蛋白同源的转录激活子，由不同时间阶段的两个不同启动子转录
作者：
A. Whitehouse;I. Carr;J. Griffiths;D. Meredith
已经鉴定了编码Epstein-Barr病毒R转录激活因子（称为ORF 50）的疱疹病毒松鼠猴（HVS）同源物的mRNA种类，并用于确定基因内的转录起始位点。第一个转录本是剪接的，起始于含有单个内含子的ORF 49内的启动子;第二个转录本是从第二个外显子内的启动子产生的，并且在相同的阅读框中。在OMK细胞中生产性病毒复制期间的早期检测到剪接的转录物，而随后检测到非剪接的转录物。剪接的转录本在激活延迟早期ORF 6启动子方面的效力是五倍;未剪接的转录本的功能尚不清楚。因此，这种蛋白质在从潜伏状态激活疱疹病毒松鼠猴中的作用可能与爱泼斯坦-巴尔病毒R蛋白显著不同。
The mRNA species encoding the herpesvirus saimiri (HVS) homolog of the Epstein-Barr virus R transcriptional activator (termed ORF50) have been identified and used to determine transcriptional start sites within the gene. The first transcript is spliced and starts from a promoter within ORF49 containing a single intron; the second is produced from a promoter within the second exon and is in the same reading frame. The spliced transcript is detected at early times during productive virus replication in OMK cells, whereas the nonspliced transcript is detected later. The spliced transcript is fivefold-more potent in activating the delayed-early ORF6 promoter; the function of the nonspliced transcript is unclear. Thus, the role of this protein in activating herpesvirus saimiri from the latent state may differ significantly from that of the Epstein-Barr virus R protein.