Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats
Effect of squalene on tissue defense system in isoproterenol-induced myocardial infarction in rats
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DOI：
10.1016/j.phrs.2004.03.004
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发表时间：
2004-09-01
影响因子：
9.3
通讯作者：
Thankappan, TK
中科院分区：
文献类型：
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作者：
Farvin, KHS;Anandan, R;Thankappan, TK
本研究旨在探讨角鲨烯对异丙肾上腺素诱导的雄性白化大鼠心肌梗死组织抗氧化状态的影响。血浆中诊断标记酶 [丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、乳酸脱氢酶 (LDH) 和肌酸磷酸激酶 (CPK)]、脂质过氧化物、还原型谷胱甘肽的水平，以及谷胱甘肽依赖性抗氧化酶 [谷胱甘肽过氧化物酶 (GPx) 和谷胱甘肽-S-转移酶 (GST)] 和抗过氧化酶的活性测定实验组大鼠心脏组织中的[过氧化氢酶(CAT)和超氧化物歧化酶(SOD)]。预先将 2% 水平的角鲨烯与饲料一起施用 45 天，可显着防止异丙肾上腺素引起的实验大鼠血浆中诊断标记酶水平的升高。角鲨烯还通过阻断脂质过氧化的诱导，对异丙肾上腺素诱导的心肌梗塞发挥抗氧化作用。还观察到防止异丙肾上腺素诱导的还原型谷胱甘肽水平以及谷胱甘肽依赖性抗氧化酶和抗过氧化酶活性改变的趋势。角鲨烯的心脏保护作用可能归因于其抗氧化特性和膜稳定作用。 (C) 2004 Elsevier Ltd. 保留所有权利。
This study was designed to examine the effects of squalene on tissue antioxidant status in isoproterenol-induced myocardial infarction in male albino rats. Levels of diagnostic marker enzymes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine phosphokinase (CPK)] in plasma, lipid peroxides, reduced glutathione, and the activities of glutathione-dependent antioxidant enzymes [glutathione peroxidase (GPx) and glutathione-S-transferase (GST)] and antiperoxidative enzymes [catalase (CAT) and superoxide dismutase (SOD)] in the heart tissue of experimental groups of rats were determined. The prior administration of squalene at 2% level along with feed for 45 days significantly prevented the isoproterenol-induced elevation in the levels of diagnostic marker enzymes in plasma of experimental rats. Squalene also exerted an antioxidant effect against isoproterenol-induced myocardial infarction by blocking the induction of lipid peroxidation. A tendency to prevent the isoproterenol-induced alterations in the level of reduced glutathione and in the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes was also observed. The cardioprotective effect of squalene might be ascribable to its antioxidant property and membrane stabilizing action. (C) 2004 Elsevier Ltd. All rights reserved.