Sexual hormones terminate in the rat: the significantly enhanced catecholaminergic/serotoninergic tone in the brain characteristic to the post-weaning period.
Sexual hormones terminate in the rat: the significantly enhanced catecholaminergic/serotoninergic tone in the brain characteristic to the post-weaning period.
复制标题
性激素在大鼠体内终止：断奶后大脑中儿茶酚胺能/血清素能张力显着增强。
DOI：
10.1016/s0024-3205(00)00671-8
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发表时间：
2000
期刊：
影响因子：
6.1
通讯作者：
János Dalló
中科院分区：
文献类型：
--
作者：
Jozsef Knoll;I. Miklya;Berta Knoll;János Dalló
从纹状体，黑质和嗅结节，去甲肾上腺素从蓝斑和5-羟色胺从中缝，释放的多巴胺的量显着高于在4和5周龄的大鼠比在3个月大的，证明儿茶酚胺能/胆碱能活性增强剂（CAE/SAE）的调节工作不受限制的发育寿命期间，并在此后受到限制。由于CAE/SAE调节的抑制（到第二个月大的时候）与性激素的出现时间一致，我们阉割了三周龄的雄性和雌性大鼠，并在它们生命的第三个月结束时测量了从选定的离散脑区域释放的儿茶酚胺和5-羟色胺。从神经元释放的儿茶酚胺和5-羟色胺的量在去势大鼠中显著高于未处理或假手术大鼠，这表明性激素抑制脑中的CAE/SAE调节。因此，我们对雄性和雌性大鼠进行s.c.处理。分别用油（0.1ml/只）、睾酮（0.1mg/只）、雌酮（0.01mg/只）和孕酮（0.5mg/只）灌胃，观察它们对CAE/SAE调节的影响。单次注射激素后24小时，去甲肾上腺素、多巴胺和5-羟色胺的释放在睾酮或雌酮治疗的大鼠中被显著抑制，但在炔雌醇治疗后保持不变。在接受单次激素注射的大鼠中，雄性的睾酮和雌性的雌酮是更有效的抑制剂。值得注意的是，在每天注射激素7天或14天的大鼠中发现了相反的效力顺序。在用激素治疗两周后，雄性中的雌酮和雌性中的睾酮是CAE/SAE调节的显著更有效的抑制剂。这些数据表明，性激素通过抑制冲动传播介导的脑中儿茶酚胺和5-羟色胺的释放来终止青春期的过度活跃阶段。
The amount of dopamine released from the striatum, substantia nigra and tuberculum olfactorium, noradrenaline from locus coeruleus and serotonin from the raphe, was significantly higher in four and five weeks old rats than in three month old ones, proving that the catecholaminergic/serotoninergic activity enhancer (CAE/SAE) regulation works unrestrained during developmental longevity and is restricted thereafter. As the dampening of the CAE/SAE regulation (end to the second month of age) coincided temporally with the appearance of sexual hormones, we castrated three weeks old male and female rats and measured at the end of the third month of their life the release of catecholamines and serotonin from selected discrete brain regions. The amount of catecholamines and serotonin released from the neurons was significantly higher in castrated than in untreated or sham operated rats, signalling that sexual hormones inhibit the CAE/SAE regulation in the brain. We therefore treated male and female rats s.c. with oil (0.1 ml/rat), testosterone, (0.1 mg/rat), estrone (0.01 mg/rat) and progesterone (0.5 mg/rat), respectively, and measured their effect on the CAE/SAE regulation. Twenty-four hours after a single injection with the hormones, the release of noradrenaline, dopamine and serotonin was significantly inhibited in the testosterone or estrone treated rats, but remained unchanged after progesteron treatment. In rats treated with a single hormone injection, testosterone in the male and estrone in the female was the significantly more effective inhibitor. Remarkably, the reverse order of potency was found in rats treated with daily hormone injections for 7 or 14 days. After two-week treatment with the hormones estrone was in the male and testosterone in the female the significantly more potent inhibitor of the CAE/SAE regulation. The data indicate that sexual hormones terminate the hyperactive phase of adolescence by dampening the impulse propagation mediated release of catecholamines and serotonin in the brain.