Administration of equol-producing bacteria alters the equol production status in the Simulator of the Gastrointestinal Microbial Ecosystem (SHIME)
Administration of equol-producing bacteria alters the equol production status in the Simulator of the Gastrointestinal Microbial Ecosystem (SHIME)
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DOI：
10.1093/jn/136.4.946
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发表时间：
2006-04-01
影响因子：
4.2
通讯作者：
Verstraete, W
中科院分区：
文献类型：
--
作者：
Decroos, K;Eeckhaut, E;Verstraete, W
大豆黄酮（大豆中主要的黄酮类化合物之一）在肠道内的微生物转化为异黄烷雌马酚具有很高的个体间变异性。后一种化合物被认为具有比其前体更高的生物活性;因此，人们对调节这种重要生物转化的饮食应用感兴趣。在2个单独的实验中，我们将我们先前分离的混合微生物培养物（EPC 4）施用到人类肠道微生物生态系统模拟器（SHIME）中，该混合微生物培养物将大豆苷元有效地转化为雌马酚。给SHIME喂食大豆胚芽粉，并接种来自两个产生雌马酚的个体的粪便样品。在两个实验中，在治疗开始后5-6天，在远端结肠隔室中诱导雌马酚产生;在中断加入EPC 4后2周，仍然大量产生雌马酚。大豆苷元在模拟结肠中的代谢存在较大的区域间差异。此外，微生物群落的组成和活性没有重大变化是由微生物聚生体的补充引起的。虽然需要在体内研究中进一步证实，但这些结果证实了施用EPC 4可以构成将壬雌酚生产者转化为生产者的新手段的概念。
The intestinal microbial transformation of daidzein, one of the principal isoflavones from soy, into the isoflavan equol is subjected to a high interindividual variability. The latter compound is considered to have a higher biological activity than its precursor; hence, there is interest in dietary applications that modulate this important biotransformation. In 2 separate experiments, we administered a mixed microbial culture (EPC4), which we had isolated previously and which efficiently transforms daidzein into equol, to the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). The SHIME was fed soy germ powder and inoculated with fecal samples from two nonequol-producing individuals. Equol production was induced in the distal colon compartments in both experiments, 5-6 d after the start of the treatment; 2 wk after interrupting the addition of EPC4, equol was still produced in high amounts. There are large interregional differences in daidzein metabolism in the simulated colon. Furthermore, no major shifts in the composition and activity of the microbial communities were caused by the supplementation with the microbial consortium. Although further confirmation in in vivo studies is required, these results validate the concept that administering EPC4 could constitute a novel means for converting a nonequol-producer into a producer.