The association of SNPs in Hsp90β gene 5′ flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds
The association of SNPs in Hsp90β gene 5′ flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds
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DOI：
10.1007/s11033-013-2630-3
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发表时间：
2013-09-01
影响因子：
2.8
通讯作者：
Luo, Qing-Bin
中科院分区：
文献类型：
--
作者：
Chen, Zhuo-Yu;Gan, Jian-Kang;Luo, Qing-Bin
热应激诱导热休克蛋白(HSPs)的表达，HSP90家族是已报道的参与细胞抗热应激保护的家族之一。但目前尚不清楚鸡体内Hsp90β基因的遗传变异是否与耐热性有关。采用直接测序法对3个鸡种(n=663)的Hsp90β基因5‘侧翼区可能存在的SNP进行了检测。在3个鸡种的Hsp90β基因上发现了6个突变，其中2个SNPs用聚合酶链式反应-限制性片段长度多态性方法进行了基因分型。关联分析表明，鸡Hsp90β基因5‘侧翼区C-141G和Gt；A的SNP对耐热性有一定的影响，可能是一个潜在的耐热性分子标记，GG为耐热型。实时定量聚合酶链式反应检测到HSP90β基因在不同组织中的表达具有组织依赖性，表明不同组织对热应激有不同的敏感性。此外，还表现出明显的时间特异性和品种差异。灵山鸡心、肝、脑、脾等组织中Hsp90β基因的表达显著高于或低于白隐性岩石鸡(WRR)。在本研究中，我们推测这些突变可用于本育种计划中抗热应激鸡的标记辅助选择，WRR对热带热应激敏感，而灵山鸡适应能力较好。
Thermo stress induces heat shock proteins (HSPs) expression and HSP90 family is one of them that has been reported to involve in cellular protection against heat stress. But whether there is any association of genetic variation in the Hsp90 beta gene in chicken with thermo tolerance is still unknown. Direct sequencing was used to detect possible SNPs in Hsp90 beta gene 5' flanking region in 3 chicken breeds (n = 663). Six mutations, among which 2 SNPs were chosen and genotypes were analyzed with PCR-RFLP method, were found in Hsp90 beta gene in these 3 chicken breeds. Association analysis indicated that SNP of C.-141G > A in the 5' flanking region of the Hsp90 beta gene in chicken had some effect on thermo tolerance traits, which may be a potential molecular marker of thermo tolerance, and the genotype GG was the thermo tolerance genotype. Hsp90 beta gene mRNA expression in different tissues detected by quantitative real-time PCR assay were demonstrated to be tissue dependent, implying that different tissues have distinct sensibilities to thermo stress. Besides, it was shown time specific and varieties differences. The expression of Hsp90 beta mRNA in Lingshan chickens in some tissues including heart, liver, brain and spleen were significantly higher or lower than that of White Recessive Rock (WRR). In this study, we presume that these mutations could be used in marker assisted selection for anti-heat stress chickens in our breeding program, and WRR were vulnerable to tropical thermo stress whereas Lingshan chickens were well adapted.