Pan2-Pan3 complex, together with Ccr4-Not complex, has a role in the cell growth on non-fermentable carbon sources
Pan2-Pan3 complex, together with Ccr4-Not complex, has a role in the cell growth on non-fermentable carbon sources
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Pan2-Pan3 复合物与 Ccr4-Not 复合物一起在不可发酵碳源上的细胞生长中发挥作用
DOI：
10.1016/j.bbrc.2021.07.007
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发表时间：
2021
影响因子：
3.1
通讯作者：
Irie Kenji
中科院分区：
文献类型：
--
作者：
Fujii Shiori;Duy Duong Long;Valderrama Arvin Lapiz;Takeuchi Risa;Matsuura Eri;Ito Ayaka;Irie Kaoru;Suda Yasuyuki;Mizuno Tomoaki;Irie Kenji
有两种主要的去腺苷酸酶复合物，Ccr 4-Not和Pan 2-Pan 3，它们缩短了mRNA的3′ poly（A）尾，并且从酵母到人类都是保守的。我们先前已经证明，Ccr 4介导的去腺苷化在酵母稳定期细胞的基因表达调控中起着重要作用。为了进一步了解失活酶在不同生长条件下的作用，在这项研究中，我们研究了两种失活酶的缺失对非发酵含碳培养基中细胞的影响。我们发现ccr 4 Δ和ccr 4 Δ pan 2 Δ突变体在YPD培养基中表现出相似的生长缺陷：当转换到含有不可发酵来源（甘油-乳酸）的培养基时，只有cr 4 Δ生长，而cr 4 Δ pan 2 Δ不生长。在GlyLac培养基中，Ccr 4、Pan 2和Pan 3均发生磷酸化，表明Ccr 4、Pan 2和Pan 3的活性可能受磷酸化的调节。为了了解Ccr 4和Pan 2在仅含非发酵来源的培养基中细胞生长中的功能，我们分离了cr 4 Δ pan 2 Δ突变体在YPGlyLac培养基上生长缺陷的多拷贝抑制子，并鉴定了两个基因STM 1和REX 2，它们分别编码核糖体相关蛋白和3′-5′ RNA外切核酸酶。我们的研究结果表明，Pan 2-Pan 3复合物，与Ccr 4-Not复合物一起，在不可发酵碳源的生长中具有重要作用。
There are two major deadenylase complexes, Ccr4-Not and Pan2-Pan3, which shorten the 3′ poly(A) tail of mRNA and are conserved from yeast to human. We have previously shown that the Ccr4-mediated deadenylation plays the important role in gene expression regulation in the yeast stationary phase cell. In order to further understand the role of deadenylases in different growth condition, in this study we investigated the effect of deletion of both deadenylases on the cell in non-fermentable carbon containing media. We found that bothccr4Δandccr4Δ pan2Δmutants showed similar growth defect in YPD media: when switched to media containing non-fermentable source (Glycerol-Lactate) only theccr4Δgrew while theccr4Δ pan2Δdid not. Ccr4, Pan2, and Pan3 were phosphorylated in GlyLac medium, suggesting that the activities of Ccr4, Pan2, and Pan3 may be regulated by phosphorylation in response to change of carbon sources. To get insights how Ccr4 and Pan2 function in the cell growth in media containing non-fermentable source only, we isolated multicopy suppressors for the growth defect on YPGlyLac media of theccr4Δ pan2Δmutant and identified two genes,STM1andREX2, which encode a ribosome-associated protein and a 3′-5′ RNA exonuclease, respectively. Our results suggest that the Pan2-Pan3 complex, together with the Ccr4-Not complex, has important roles in the growth on non-fermentable carbon sources.