The microfluidic bioreactor for a new era of bioprocess development
The microfluidic bioreactor for a new era of bioprocess development
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DOI：
10.1002/elsc.201500026
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发表时间：
2016-03-01
影响因子：
2.7
通讯作者：
Frische, Niklas
中科院分区：
文献类型：
--
作者：
Blesken, Christian;Olfers, Till;Frische, Niklas
来自m2 p-labs GmbH的BioLector（R）Pro系统使用具有集成微流体芯片的微量滴定板（MTP）。通过使用微流控技术，该系统可以成功地进行小规模的流加培养。使用0.8-1.5 mL的工作体积进行培养，因为它们仅可能在实验室发酵罐中进行。生物量、荧光、pH和溶解氧的测量通过非侵入式光学方法进行。pH和进料速率的控制通过微阀和微通道实现。这些独特的微流体组件首次在MTP格式上实现了连续进料和pH控制。总共将32个生物反应器威尔斯孔和16个储存器威尔斯孔放置在一个板上。这意味着32个补料分批培养可以并行运行，完全自动化，并具有广泛的数据输出。
The BioLector (R) Pro system from m2p-labs GmbH uses microtiter plates (MTPs) with an integrated microfluidic chip. By using microfluidic technology, the system can successfully carry out small-scale fed-batch cultivations. Working volumes of 0.8-1.5 mL are used to conduct cultivations as they were only as yet possible in lab fermenters. The measurements of biomass, fluorescence, pH and dissolved oxygen are performed by non-invasive optical methods. The control of pH and feeding rates are realized by micro-valves and micro-channels. For the first time, these unique microfluidic components achieve continuous feeding and pH control on an MTP format. Altogether, 32 bioreactor wells and 16 reservoir wells are placed on one plate. That means 32 fed-batch cultivations can be run in parallel, completely automated, with extensive data output.