Comparison between thaw-mounting and use of conductive tape for sample preparation in ToF-SIMS imaging of lipids in Drosophila microRNA-14 model
Comparison between thaw-mounting and use of conductive tape for sample preparation in ToF-SIMS imaging of lipids in Drosophila microRNA-14 model
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DOI：
10.1116/1.5019597
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发表时间：
2018-05-01
期刊：
影响因子：
2.1
通讯作者：
Lee, Tae Geol
中科院分区：
文献类型：
--
作者：
Le, Minh Uyen Thi;Son, Jin Gyeong;Lee, Tae Geol
飞行时间二次离子质谱（ToF-SIMS）成像阐明了组织切片中的分子分布，提供了与疾病相关的代谢途径的有用信息。然而，在样品制备过程中的分析物的离域和不充分的组织粘附是与这种技术相关的一些不幸的现象，由于它们在降低ToF-SIMS图像的质量，可靠性和空间分辨率中的作用。由于这些原因，ToF-SIMS成像需要更严格的样品制备方法，以保持组织的自然状态。传统的解冻安装方法特别容易受到由于热效应以及组织收缩引起的分析物分布改变的影响。在本研究中，作者比较了不同的组织封片方法，包括解冻封片方法。作者使用导电胶带作为基底上的组织固定材料，因为它不需要手指的热量来使组织切片粘附到基底上，并且可以减少数据采集期间的电荷积累。通过导电胶带取样方法，他们能够获得可重复的组织切片和高质量的图像，而不会重新分配分子。此外，作者通过在microRNA-14（miR 14）缺失的果蝇模型中使用磁带支持的采样，成功地保留了脂肪代谢物（如二酰基甘油和脂肪酸）的不同组分的自然状态和化学分布。这里强调的方法显示了组织样品质谱成像准确性的提高。(C)2018年作者。所有文章内容，除非另有说明，是根据知识共享署名（CC BY）许可证许可。
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging elucidates molecular distributions in tissue sections, providing useful information about the metabolic pathways linked to diseases. However, delocalization of the analytes and inadequate tissue adherence during sample preparation are among some of the unfortunate phenomena associated with this technique due to their role in the reduction of the quality, reliability, and spatial resolution of the ToF-SIMS images. For these reasons, ToF-SIMS imaging requires a more rigorous sample preparation method in order to preserve the natural state of the tissues. The traditional thaw-mounting method is particularly vulnerable to altered distributions of the analytes due to thermal effects, as well as to tissue shrinkage. In the present study, the authors made comparisons of different tissue mounting methods, including the thaw-mounting method. The authors used conductive tape as the tissue-mounting material on the substrate because it does not require heat from the finger for the tissue section to adhere to the substrate and can reduce charge accumulation during data acquisition. With the conductive-tape sampling method, they were able to acquire reproducible tissue sections and highquality images without redistribution of the molecules. Also, the authors were successful in preserving the natural states and chemical distributions of the different components of fat metabolites such as diacylglycerol and fatty acids by using the tape-supported sampling in microRNA-14 (miR14) deleted Drosophila models. The method highlighted here shows an improvement in the accuracy of mass spectrometric imaging of tissue samples. (C) 2018 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license.