EXPRESSION OF THE NORMAL HUMAN SIS/PDGF-2 CODING SEQUENCE INDUCES CELLULAR-TRANSFORMATION
EXPRESSION OF THE NORMAL HUMAN SIS/PDGF-2 CODING SEQUENCE INDUCES CELLULAR-TRANSFORMATION
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DOI：
10.1016/0092-8674(84)90194-6
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
AARONSON, SA
中科院分区：
文献类型：
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作者：
GAZIT, A;IGARASHI, H;AARONSON, SA
人 sis 原癌基因包含生物活性人血小板衍生生长因子 (PDGF) 制剂中存在的 2 条多肽链中 1 条的编码序列。人类克隆 c-sis 克隆 8 包含人类 DNA 中存在的所有 v-sis 相关序列，当转染到 NIH/3T3 细胞中时，转录处于失活状态。当置于逆转录病毒LTR的控制下时，克隆的转录水平与在SSV DNA转化的细胞中观察到的水平相当。 c-sis克隆8 DNA不表达可检测的sis/PDGF-2蛋白并且缺乏生物活性。假定的上游外显子通过其在某些人类细胞中检测 4.2 kilobase sis 相关转录物的能力而被鉴定。当该序列以正确方向插入LTR和c-sis克隆8之间时，嵌合分子获得了与SSV DNA相当的高滴度转化活性。含有该构建体的转化体表达人sis/PDGF-2翻译产物。当在适当的测定细胞中表达时，人生长因子的数字编码序列具有转化活性。
The human sis proto-oncogene contains the coding sequence for 1 of 2 polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH/3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kilobase sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. The number coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.