Sequence and cultivation study of Muribaculaceae reveals novel species, host preference, and functional potential of this yet undescribed family
Sequence and cultivation study of Muribaculaceae reveals novel species, host preference, and functional potential of this yet undescribed family
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DOI：
10.1186/s40168-019-0637-2
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发表时间：
2019-02-19
期刊：
影响因子：
15.5
通讯作者：
Clavel, Thomas
中科院分区：
文献类型：
--
作者：
Lagkouvardos, Ilias;Lesker, Till R.;Clavel, Thomas
S24-7(拟杆菌门)中的细菌在小鼠肠道微生物区系中占主导地位，在其他动物的肠道中也有发现。方法通过对S24-7进行大规模16S rRNA基因分析，结合后基因组和培养物种的功能和分类学研究数据，对S24-7家族进行了研究。虽然许多物种不能被分配为生态栖息地(分析了93,045个样本)，但老鼠是最常见的宿主(平均相对丰度为20%，共存在9个物种)。鸟枪式元基因组学允许重建59个分子物种，其中34个代表16S rRNA基因衍生的物种簇。此外，培养工作还分离出了代表三个物种的五个菌株，其中包括两个新的分类群。基因组分析表明，S24-7 spp.结论我们为S24-7细菌家族的多样性、生态学和描述提供了新的资料，建议将其命名为小鼠李杆菌科。
BackgroundBacteria within family S24-7 (phylum Bacteroidetes) are dominant in the mouse gut microbiota and detected in the intestine of other animals. Because they had not been cultured until recently and the family classification is still ambiguous, interaction with their host was difficult to study and confusion still exists regarding sequence data annotation.MethodsWe investigated family S24-7 by combining data from large-scale 16S rRNA gene analysis and from functional and taxonomic studies of metagenomic and cultured species.ResultsA total of 685 species was inferred by full-length 16S rRNA gene sequence clustering. While many species could not be assigned ecological habitats (93,045 samples analyzed), the mouse was the most commonly identified host (average of 20% relative abundance and nine co-occurring species). Shotgun metagenomics allowed reconstruction of 59 molecular species, of which 34 were representative of the 16S rRNA gene-derived species clusters. In addition, cultivation efforts allowed isolating five strains representing three species, including two novel taxa. Genome analysis revealed that S24-7 spp. are functionally distinct from neighboring families and versatile with respect to complex carbohydrate degradation.ConclusionsWe provide novel data on the diversity, ecology, and description of bacterial family S24-7, for which the name Muribaculaceae is proposed.