Possible Involvement of Infection With Human Coronavirus 229E, but not NL63, in Kawasaki Disease
Possible Involvement of Infection With Human Coronavirus 229E, but not NL63, in Kawasaki Disease
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DOI：
10.1002/jmv.23950
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发表时间：
2014-12-01
影响因子：
12.7
通讯作者：
Taguchi, Fumihiro
中科院分区：
文献类型：
--
作者：
Shirato, Kazuya;Imada, Yoshio;Taguchi, Fumihiro
虽然RT-PCR分析曾认为人冠状病毒(HCoV)-NL63可能是川崎病的病原体，但后续研究无法证实这一结果。在本研究中，通过血清学试验探讨了这种可能性。为了评估HCoV感染在川崎病患者中的作用，进行了免疫荧光试验和病毒中和试验。配对血清样本来自未接受-球蛋白治疗的川崎病患者。以HCoV-NL63和两个抗原性不同的HCoV-229E分离株（ATCC-VR740和新分离株仙台- h）作为对照。免疫荧光法检测川崎病患者与对照组HCoV-NL63抗体阳性率无差异，而HCoV-229E抗体阳性率川崎病患者高于对照组。中和试验显示川崎病患者急性期和恢复期两种hcov - 229e血清阳性无差异。然而，与对照组相比，从恢复期患者获得的川崎病标本显示仙台- h阳性率显著升高，但ATCC-VR740阳性率不高。血清学检测不支持HCoV-NL63参与，但提示HCoV-229E可能参与川崎病的发展。中华医学杂志，2014,36(6):556 - 556。(c) 2014 Wiley期刊公司
Although human coronavirus (HCoV)-NL63 was once considered a possible causative agent of Kawasaki disease based on RT-PCR analyses, subsequent studies could not confirm the result. In this study, this possibility was explored using serological tests. To evaluate the role of HCoV infection in patients with Kawasaki disease, immunofluorescence assays and virus neutralizing tests were performed. Paired serum samples were obtained from patients with Kawasaki disease who had not been treated with -globulin. HCoV-NL63 and two antigenically different isolates of HCoV-229E (ATCC-VR740 and a new isolate, Sendai-H) were examined as controls. Immunofluorescence assays detected no difference in HCoV-NL63 antibody positivity between the patients with Kawasaki disease and controls, whereas the rate of HCoV-229E antibody positivity was higher in the patients with Kawasaki disease than that in controls. The neutralizing tests revealed no difference in seropositivity between the acute and recovery phases of patients with Kawasaki disease for the two HCoV-229Es. However, the Kawasaki disease specimens obtained from patients in recovery phase displayed significantly higher positivity for Sendai-H, but not for ATCC-VR740, as compared to the controls. The serological test supported no involvement of HCoV-NL63 but suggested the possible involvement of HCoV-229E in the development of Kawasaki disease. J. Med. Virol. 86:2146-2153, 2014. (c) 2014 Wiley Periodicals, Inc.