The role of calmodulin in rat parotid amylase secretion: effects of calmodulin antagonists on secretion and acinar cell structure.
The role of calmodulin in rat parotid amylase secretion: effects of calmodulin antagonists on secretion and acinar cell structure.
复制标题
钙调蛋白在大鼠腮腺淀粉酶分泌中的作用：钙调蛋白拮抗剂对分泌和腺泡细胞结构的影响。
DOI：
10.1254/jjp.50.149
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Kanazawa
中科院分区：
文献类型：
--
作者：
Y. Tojyo;Y. Matsumoto;K. Okumura;M. Kanazawa
采用大鼠腮腺细胞，研究了三种钙调素拮抗剂三氟拉嗪（TFP）、N-（6-氨基己基）-5-氯-1-萘磺酰胺（W-7）和N-（6-氨基己基）-1-萘磺酰胺（W-5）对腺泡细胞淀粉酶释放和结构的影响。TFP和W-7强烈抑制异丙肾上腺素（ISO）和二丁酰环AMP刺激的淀粉酶释放在50或100 μ M的浓度，而W-5，弱钙调素拮抗剂，只有很小的影响。即使在TFP或W-7的存在下，ISO也能显著升高环AMP水平。这些结果表明，钙调素拮抗剂影响淀粉酶释放的步骤远端环AMP代谢。电子显微镜照片表明，无论是TFP或W-7治疗腮腺细胞引起的管腔微绒毛和表面褶皱的损失。当细胞在TFP或W-7存在下被ISO刺激时，扩大的管腔不能恢复到其原始大小，并且排出的分泌物质保留在管腔中。腺泡胞浆内有大量分泌颗粒。W-5对腺泡细胞结构影响较小。这些观察结果导致TFP和W-7干扰细胞骨架系统的正常功能的假设。提示钙调素可能通过调节细胞骨架系统参与腮腺淀粉酶的胞吐作用。
Using dispersed rat parotid cells, the effects of three calmodulin antagonists, trifluoperazine (TFP), N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide (W-7), and N-(6-aminohexyl)-1-naphthalenesulfonamide (W-5), on amylase release and acinar cell structure were examined. TFP and W-7 strongly inhibited both isoproterenol (ISO)- and dibutyryl cyclic AMP-stimulated amylase release at a concentration of 50 or 100 microM, while W-5, a weak calmodulin antagonist, had only little effect. Cyclic AMP level was markedly elevated by ISO even in the presence of TFP or W-7. These results indicate that the calmodulin antagonists affect amylase release at steps distal to cyclic AMP metabolism. Electron micrographs demonstrated that treatment of parotid cells with either TFP or W-7 caused a loss of luminal microvilli and surface folds. When cells were stimulated by ISO in the presence of TFP or W-7, the enlarged lumina did not recover to their original size and the discharged secretory material was retained in the lumina. Numerous secretory granules remained in the acinar cytoplasm. W-5 affected the acinar cell structure only a little. These observations lead to the assumption that TFP and W-7 interfered with the normal functions of the cytoskeletal system. It is proposed that calmodulin may be involved in the exocytosis of parotid amylase through the regulation of the cytoskeletal system.