Composition and density of bacterial biofilms determine larval settlement of the polychaete Hydroides elegans
Composition and density of bacterial biofilms determine larval settlement of the polychaete Hydroides elegans
复制标题
DOI：
10.3354/meps260161
复制
发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
Hadfield, MG
中科院分区：
文献类型：
--
作者：
Huang, SY;Hadfield, MG
多毛类蠕虫Hydroides elegans的幼虫在海洋生物膜的作用下沉降和变形。4种海洋生物膜细菌16SrDNA序列的系统发育关系不能预测其诱导能力。两个相距30%遗传距离的菌株在诱导H.优美的两个细菌菌株的16S rRNA基因序列只有3%的分歧是非常不同的，一个菌株诱导变态强烈，而另一个没有。无论是天然生物膜还是由单一细菌组成的生物膜，变态幼虫的百分比与细菌密度呈正相关。当比较4种细菌物种的测试结果时，最具诱导性的细菌物种在比弱或无诱导性细菌低得多的生物膜密度下有效。本文的证据表明，触发黑腹潜蝇幼虫定居和变态的信号是一种重要的信号。秀丽线虫不是单一细菌分类群所特有的，并且可能是不溶性的表面结合材料。
Larvae of the polychaete worm Hydroides elegans settle and metamorphose in response to marine biofilms. Phylogenetic relationships among 4 marine-biofilm bacterial species identified by 16S rDNA sequences were not predictive of their inductive capacity. Two bacterial species separated by a genetic distance of 30 % shared the greatest capacity for inducing metamorphosis in H. elegans. Two bacterial strains with only a 3 % divergence in 16S rRNA gene sequences were very different; one strain induced metamorphosis strongly while the other one did not. The percent of larvae that metamorphosed correlated positively with bacterial density in either natural biofilms or biofilms composed of a single bacterial species. When results of tests across 4 bacterial species were compared, the most inductive bacterial species was effective in much lower biofilm densities than weakly or non-inductive bacteria. Evidence presented here indicates that the cue that triggers settlement and metamorphosis in larvae of H. elegans is not unique to a single bacterial taxon and is likely to be an insoluble, surface-bound material.