Identification of specific high affinity sites for the estradiol receptor in the erythrocyte cytoskeleton.
Identification of specific high affinity sites for the estradiol receptor in the erythrocyte cytoskeleton.
复制标题
红细胞骨架中雌二醇受体特异性高亲和力位点的鉴定。
DOI：
10.1016/0006-291x(81)90504-0
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发表时间：
1981
影响因子：
3.1
通讯作者：
Vincenzo Sica
中科院分区：
文献类型：
--
作者：
G. Puca;Vincenzo Sica
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The possible relationship of the soluble, “cytosolic” estradiol receptor with complex membranous and cytoskeletal structures of the cell matrix has been studied using a model erythrocyte system. Extraction of erythrocyte ghosts with a nonionic detergent (Triton X-100) under conditions that yield a cytoskeletal matrix reveals the presence of a limited number (less than 100) of specific sites having high affinity (kd10−9M) for the estradiol-receptor complex. The interation between the estradiol receptor and the cytoskeleton is critically dependent on temperature and it is improved by 25 mM KCl or NaCl and by 2.5 mM MgCl2. The data suggest that the estradiol receptor, which has been generally considered to be freely “soluble” in the cytoplasm, may actually be physiologically associated in an integral manner with a complex cytoskeletal network in the cell cytoplasm.