Preclinical methods for the evaluation of periodontal regeneration in vivo.
Preclinical methods for the evaluation of periodontal regeneration in vivo.
复制标题
DOI：
10.1007/978-1-60761-820-1_18
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Giannobile WV
中科院分区：
文献类型：
--
作者：
Seol YJ;Pellegrini G;Franco LM;Chang PC;Park CH;Giannobile WV
为了确定促进牙周再生的关键因素或装置，使用活体动物模型进行临床前调查是在人类临床试验测试之前评估生物学反应的关键。在这一章中，我们提供了常用的临床前动物的研究，以促进骨和软组织修复牙齿支持的牙周缺损骨和软组织重建程序的研究。提供了动物管理的步骤，用于使用描述性组织学、组织形态计量学、三维成像和安全性评估来评估结果测量。使用这些牙周再生的关键措施应该有助于研究人员选择合适的替代终点用于临床，这些终点在人类身上是不实用或不道德的。这些方法将使研究人员做好准备，并帮助他们确定终点，然后这些终点可以适应人类临床试验计划。
For the determination of key factors or devices that promote periodontal regeneration, preclinical investigations using in vivo animal models are critical for evaluating the biological responses before human clinical trial testing. In this chapter, we provide an overview on the commonly used preclinical animals for the study of reconstructive procedures to promote bone and soft tissue repair of tooth-supporting periodontal defects. Steps are provided on the animal management for evaluation of outcome measures using descriptive histology, histomorphometry, three-dimensional imaging, and safety assessments. The use of these key measures of periodontal regeneration should aid investigators in the selection of appropriate surrogate endpoints to be utilized in the clinical arena, which are not practical or ethical in humans. These methods will prepare investigators and assist them in identifying endpoints that can then be adapted to human clinical trial planning.