Development of an implantable muscle stimulator: Measurement of stimulated angiogenesis and poststimulus vessel regression
Development of an implantable muscle stimulator: Measurement of stimulated angiogenesis and poststimulus vessel regression
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DOI：
10.1038/sj.mn.7300100
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发表时间：
2000-04-01
期刊：
影响因子：
2.4
通讯作者：
Greene, AS
中科院分区：
文献类型：
--
作者：
Linderman, JR;Kloehn, MR;Greene, AS
目的：研制一种轻便、可植入的骨骼肌电刺激器。刺激器可以被编程为在给定的时间段内以全自动模式运行不同的开-关间隔。使用的刺激，血管生成，促进以研究在刺激remove.Methods后，血管生长和随后的回归发生的速度：一个完全植入式数字刺激器的设计和制造。将刺激器皮下嵌入雄性Sprague-Dawley大鼠的胸腰椎区域，并将电极在皮肤下穿隧至右后肢的腓总神经。结果：慢性刺激腓总神经支配的骨骼肌，可引起胫前肌（TA：26%）和趾长伸肌的血管密度显著增加，其中胫前肌（TA：26%）和趾长伸肌（TA：26%）的血管密度显著增加（EDL; 19%，7天内。血管密度在刺激后第3天和第7天仍然升高。结论：nem刺激器可在7天内促进血管密度的显著增加，使刺激血管生长和刺激后稀疏的研究成为可能。由于其体积小和可靠的计时周期，该刺激器应被证明是研究这些现象的有价值的工具。
Objective: We developed a lightweight, totally implantable electrical stimulator designed to elicit contraction of skeletal muscle. The stimulator can be programmed to run for different on-off intervals in a given time period in a fully automatic mode. Using the stimulator, angiogenesis, was promoted in order to study the rate at which vessel growth and subsequent regression occurs after stimulus removal.Methods: A fully implanted digital stimulator was designed and fabricated. The stimulator was embedded subcutaneously in the thoracolumbar region of male Sprague-Dawley rats and the electrodes were tunneled under the skin to the common peroneal nerve of the right hind limb. The stimulator elicited muscle contraction in the hind limb at 10 s(-1) using square-wave pulses 0.3 ms in duration, evoking contraction of specific muscles for 8 hours/day for 7 days.Results: Chronic stimulation of the skeletal muscles innervated by the common peroneal nerve led to significant increases in blood vessel density in the tibialis anterior (TA: 26%) and the extensor digitorum longus (EDL; 19%) within 7 days. The vessel density remained elevated at 3 days and 7 days poststimulation. but subsequently decreased to control levels by 14 days poststimulation.Conclusion: The nem stimulator can promote significant increases in vessel density within 7 days, allowing study of both stimulated vessel growth and poststimulus rarefaction, Because of its small size and reliable timings cycles, the stimulator should prove to be a valuable tool in studying these phenomena.