Age-Associated Different Transcriptome Profiling in Zebrafish and Rats: an Insight into the Diversity of Vertebrate Aging
Age-Associated Different Transcriptome Profiling in Zebrafish and Rats: an Insight into the Diversity of Vertebrate Aging
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DOI：
10.1007/s10126-022-10153-9
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发表时间：
2022-09
影响因子：
3
通讯作者：
Yusuke Kijima;Wantong Wang;Yoji Igarashi;K. Yoshitake;S. Asakawa;Yutaka Suzuki;S. Watabe;S. Kinoshita
中科院分区：
文献类型：
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作者：
Yusuke Kijima;Wantong Wang;Yoji Igarashi;K. Yoshitake;S. Asakawa;Yutaka Suzuki;S. Watabe;S. Kinoshita
大多数哺乳动物，包括人类，都表现出明显的衰老表型，例如组织可塑性丧失和骨质疏松症。在这方面，鱼类由于其独特的衰老特性，可以成为研究衰老的有吸引力的模型。鱼的寿命差异很大，有几个物种可以活到200多年。此外，一些鱼在一生中表现出抗衰老和不确定的生长特性。因此，探讨鱼类的衰老机制可以为研究脊椎动物的衰老提供新的视角。为此，我们对斑马鱼的不同器官和生长阶段进行了RNA测序(RNA-seq)分析，并将数据与之前发表的大鼠RNA-seq数据进行了比较。所有斑马鱼组织样本的年龄相关差异表达基因(Degs)揭示了昼夜节律基因的上调和hmgb3a的下调。一方面，斑马鱼和大鼠之间与衰老相关的DEG图谱的比较分析发现，昼夜节律基因的上调和胶原基因的下调是保守的转录组变化。另一方面，在斑马鱼中，观察到肌肉中自噬相关基因和各种组织中AP-1转录因子基因的上调，这可能暗示了鱼类特有的抗衰老特性。与我们对哺乳动物衰老的了解一致，在大鼠身上观察到了与组织衰老相关的DEG谱。我们还检测到斑马鱼鳃中与年龄相关的肌肉动态平衡和分化相关基因的下调，表明斑马鱼特有的衰老表型。我们的结果表明，鱼类和哺乳动物之间既有共同的衰老特征，也有不同的衰老特征，这可能会被用于未来的翻译研究。
Most mammals, including humans, show obvious aging phenotypes, for example, loss of tissue plasticity and sarcopenia. In this regard, fish can be attractive models to study senescence because of their unique aging characteristics. The lifespan of fish varies widely, and several species can live for over 200 years. Moreover, some fish show anti-aging features and indeterminate growth throughout their life. Therefore, exploring the aging mechanism in fish could provide new insights into vertebrate aging. To this end, we conducted RNA sequencing (RNA-seq) assays for various organs and growth stages of zebrafish and compared the data with previously published RNA-seq data of rats. Age-associated differentially expressed genes (DEGs) for all zebrafish tissue samples reveal the upregulation of circadian genes and downregulation ofhmgb3a. On one hand, a comparative analysis of DEG profiles associated with aging between zebrafish and rats identifies upregulation of circadian genes and downregulation of collagen genes as conserved transcriptome changes. On the other hand, in zebrafish, upregulation of autophagy-related genes in muscles and AP-1 transcription factor genes in various tissues is observed, which may imply fish-specific anti-aging characteristics. Consistent with our knowledge of mammalian aging, DEG profiles related to tissue senescence are observed in rats. We also detect age-associated downregulation of muscle homeostasis and differentiation-related genes in zebrafish gills, indicating a fish-specific senescence phenotype. Our results indicate both common and different aging profiles between fish and mammals, which could be used for future translational research.