Corynebacterium phoceense, resident member of the urogenital microbiota?
Corynebacterium phoceense, resident member of the urogenital microbiota?
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DOI：
10.1099/mic.0.001068
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发表时间：
2021-07-01
期刊：
影响因子：
2.8
通讯作者：
Putonti, Catherine
中科院分区：
文献类型：
--
作者：
Giannattasio-Ferraz, Silvia;Ene, Adriana;Putonti, Catherine
Phoceense棒状杆菌是先前从人尿液中分离的革兰氏阳性菌种。虽然来自同一属的其他物种与尿路感染有关，但C。Phoceense目前被认为是泌尿生殖器微生物群的非致病性成员。在我们的研究之前，文献中只描述了两个分离株，对该物种知之甚少。在这里，我们描述C。phoceense UFMG-H7，是从健康牛尿中分离到的该种的第一株。该分离物的基因组被产生并与另外两个公开可用的C. phoceense以及其他棒状杆菌基因组组装体。我们的深入基因组分析鉴定了另外四种公开可用的基因组组装体，它们是该物种的代表，也从人类泌尿生殖道分离。虽然没有一个菌株与症状或疾病相关，但编码了许多与毒力因子相关的基因。与相关的棒状杆菌属种和来自牛阴道的棒状杆菌属种相反，所有的C. phoceense菌株检测的SpaD型皮利编码表明粘附对于其在泌尿道内的持久性是必需的。作为另一个C.分析的phoceense菌株是从人类泌尿生殖道分离的，我们的结果表明，该物种可能是该生态位的特异性。
Corynebacterium phoceense is a Gram-positive species previously isolated from human urine. Although other species from the same genus have been associated with urinary tract infections, C. phoceense is currently believed to be a non-pathogenic member of the urogenital microbiota. Prior to our study, only two isolates were described in the literature, and very little is known about the species. Here, we describe C. phoceense UFMG- H7, the first strain of this species isolated from the urine of healthy cattle. The genome for this isolate was produced and compared to the two other publicly available C. phoceense as well as other Corynebacterium genome assemblies. Our in -depth genomic analysis identified four additional publicly available genome assemblies that are representatives of the species, also isolated from the human urogenital tract. Although none of the strains have been associated with symptoms or disease, numerous genes associated with virulence factors are encoded. In contrast to related Corynebacterium species and Corynebacterium species from the bovine vaginal tract, all C. phoceense strains examined code for the SpaD- type pili suggesting adherence is essential for its persistence within the urinary tract. As the other C. phoceense strains analysed were isolated from the human urogenital tract, our results suggest that this species may be specific to this niche.