Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation
Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation
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DOI：
10.1088/2053-1591/3/7/075404
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发表时间：
2016-07-01
影响因子：
2.3
通讯作者：
Rocha, Fernando
中科院分区：
文献类型：
--
作者：
Castro, Filipa;Ribeiro, Viviana P.;Rocha, Fernando
本文报道了在放大介观振荡流反应器(Meso-OFR)中用连续流沉淀法合成的纳米羟基磷灰石(HAP)的生物学评价。对合成的羟基磷灰石进行了物化表征，结果表明，由于其高比表面积和低结晶度，具有较高的表面反应性。另一方面，体外生物矿化分析表明，与商品化的HAP相比，所制备的HAP具有形成磷灰石的活性和更高的表面反应性。此外，人成骨样细胞(Saos-2)培养表明，合成的HAP可刺激细胞增殖，尤其是在较低浓度(30和50mUg·ml(-1))时，尽管其细胞摄取行为。因此，制备的羟基磷灰石在生物医用材料、药物和基因载体等方面显示出巨大的潜力。由于设计的方法允许以连续模式制备具有受控物理化学性质的纳米羟基磷灰石，因此该结果在进一步扩大工艺规模方面也是非常有希望的。
This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HAp suggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 mu g ml(-1)), although its cellular uptake behavior. Therefore, the prepared HAp shows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.