Escort cells generate a dynamic compartment for germline stem cell differentiation via combined Stat and Erk signalling
Escort cells generate a dynamic compartment for germline stem cell differentiation via combined Stat and Erk signalling
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DOI：
10.1242/dev.143727
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发表时间：
2017-06-01
期刊：
影响因子：
4.6
通讯作者：
Gilboa, Lilach
中科院分区：
文献类型：
--
作者：
Banisch, Torsten U.;Maimon, Iris;Gilboa, Lilach
两个不同的隔室支持生殖系干细胞（GSC）的自我更新和及时分化：经典的生态位提供维护线索，而分化隔室，体细胞护送细胞（EC）形成的，是需要适当的GSC分化。EC延伸长突起，侵入紧密堆积的生殖细胞之间，并在封装和释放它们之间交替。EC如何实现这种动态平衡尚未解决。通过结合果蝇的实时成像和遗传分析，我们已经表征了EC形状及其动态变化。我们发现，生殖细胞的EC封装是一个共同的现象，从而EC-EC接触稳定了广泛的网状突起。我们进一步表明，信号转导子和转录激活子（Stat）和表皮生长因子受体（Egfr）信号传导通过组合影响不同RhoGTP酶的活性来维持EC的灵活性和灵活性。我们的研究结果揭示了复杂的信号网络如何决定细胞的形状及其动态行为。这也解释了分化区室如何与生殖细胞建立广泛的接触，同时允许分化的GSC子细胞持续向后移动。
Two different compartments support germline stem cell (GSC) self-renewal and their timely differentiation: the classical niche provides maintenance cues, while a differentiation compartment, formed by somatic escort cells (ECs), is required for proper GSC differentiation. ECs extend long protrusions that invade between tightly packed germ cells, and alternate between encapsulating and releasing them. How ECs achieve this dynamic balance has not been resolved. By combining live imaging and genetic analyses in Drosophila, we have characterised EC shapes and their dynamic changes. We show that germ cell encapsulation by ECs is a communal phenomenon, whereby EC-EC contacts stabilise an extensive meshwork of protrusions. We further show that Signal Transducer and Activator of Transcription (Stat) and Epidermal Growth Factor Receptor (Egfr) signalling sustain EC protrusiveness and flexibility by combinatorially affecting the activity of different RhoGTPases. Our results reveal how a complex signalling network can determine the shape of a cell and its dynamic behaviour. It also explains how the differentiation compartment can establish extensive contacts with germ cells, while allowing a continual posterior movement of differentiating GSC daughters.